Aspects of Hematite Bioflotation Using a Gram-Positive Bacillus Subtilis Strain as a Bioreagent

The recent literature has unveiled the potential use of microbial strains in mineral bioprocessing. This study presents the use of a Gram-positive bacterium (Bacillus subtilis) as bioreagent for hematite flotation. The bacterium presents the ability to modify the properties of mineral surface (electrophoretic mobility and hydrophobicity). It was studied the electrokinetic behavior of hematite particles, before and after the interaction with the bacterial cells. The results revealed a shift of the isoelectric point (IEP) of the hematite after interaction with bacterial cells, suggesting a chemical adsorption mechanism. Moreover, after bacterial conditioning, the zeta potential curve of hematite presented high values in the alkaline pH range. The hematite and the bacterial surfaces presented contact angle values around 27.4° and 41.0°, respectively. According to the results, the B. subtilis strain was capable of modifying the hematite surface, and after interaction the hematite surface presented a contact angle value around 46.0°. The flotation tests were carried out in a modified Hallimond tube. The experiments revealed a significant increase in hematite flotability using the Gram-positive strain as bioreagent. The tests pointed out a maximum recovery of hematite in the presence of 600 mg/L and pH 5. Therefore, the results from this study showed the potential use of the B. subtilis strain as a biocollector for hematite flotation, and its possible application in the mineral flotation industry.