Can Bacteriophage be Used to Separate Minerals?

Bacteriophage (phage) are viruses that infect bacteria. Protein coatings on the surface of the phage particle can be genetically engineered so that they bind to inorganic molecules. The filamentous phage particle is about 930 nanometres in length and 6.5 nanometres in diameter and consists of a single-stranded molecule of DNA surrounded by five different coat protein molecules denoted P3, P6, P7, P8 and P9. Peptide sequences in the P3 coat protein that bind to sphalerite and chalcopyrite have been identified. The binding is selective; phage with specific P3 peptide sequences bind to minerals but do not bind to silica or to pyrite. Two peptide sequences in the P8 coat protein that bind selectively to chalcopyrite and pyrite have also been identified. The amino acids in the P8 binding sequences are hydrophilic and thus a coating of a mineral particle with phage having these peptide sequences may render the particle hydrophilic. Experiments are underway to confirm this. Phage are relatively easy to grow and are not living organisms requiring an energy source to maintain life. Phage can also tolerate a range of pH and temperatures. Given this, and the ability to indentify phage coat proteins that bind selectively to a variety of inorganic molecules, phage have the potential to be useful tools in mineral processing and waste treatment.