Carbonic Anhydrase And Carboxypeptidase A Metalloprotein Affinity Metal Chromatography

Previous efforts to remove and concentrate heavy-metal ions from wastewater streams via metalloprotein affinity metal chromatography (MAMC) were limited to the metal transport protein transferrin. Extension of the technique to the enzymes carbonic anhydrase and carboxypeptidase A, which possess different metal affinity properties, is described. Preliminary results indicate that the technique is capable of selectively removing and separating late first-row transition metal ions from aqueous solution. The results suggest that by properly choosing the matrix, i.e., protein or enzyme, MAMC may he applicable to problems involving a wide range of heavy-metal-hearing aqueous solutions.