Investigation of Intermediate Species and Electrochemical Dissolution of Chalcopyrite in the Presence of Sulfur and Iron Oxidizing Microorganisms

"In the present work, X-ray diffraction (XRD) analysis, X-ray photoelectron spectroscopy (XPS) analysis and electrochemical analysis were carried out to investigate the intermediate species and electrochemical dissolution process of chalcopyrite in the presence of sulfur and iron oxidizing microorganisms. For bioleaching of chalcopyrite by the sulfur oxidizing microorganism Acidithiobacillus caldus, the redox potential was always lower than the appropriate value and chalcopyrite was mainly directly oxidized to polysulfide (Sn 2-), thus resulting in low copper extraction. As for bioleaching of chalcopyrite by the iron oxidizing microorganism Leptospirillum ferriphilum, chalcopyrite was mainly directly oxidized to polysulfide at the initial stage where redox potential was lower than the appropriate value and resulted in low dissolution rate. At the middle stage where redox potential was appropriate (about 350–480 mV vs. Ag/AgCl), chalcopyrite was mainly transformed to intermediate species of Cu2S rather than polysulfide, thus resulting in high dissolution rate. At the later stage where redox potential was higher than the appropriate value, chalcopyrite was mainly directly oxidized to polysulfide which caused the later passivation of chalcopyrite. Finally, a model of electrochemical dissolution process of chalcopyrite in the presence of sulfur and iron oxidizing microorganisms was provided.INTRODUCTIONBiohydrometallurgy is considered as one promising mineral processing technique in processing low grade ores due to the advantages of simplicity, low cost and eco-friendly. Chalcopyrite (CuFeS2) is the most abundant copper-bearing resource, accounting for appropriately 70% of the known reserves in the world, while is recalcitrant to bio-hydrometallurgy mainly due to the coated passivation layer (Klauber, 2008; Li, Kawashima, Li, Chandra, & Gerson, 2013). However, the detailed dissolution and passivation mechanisms of chalcopyrite bioleaching are still uncertain. The microorganisms assisting chalcopyrite dissolution can be mainly classified into three types, including mesophilic microorganisms (e.g., Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Leptospirillum ferrooxidans), moderately thermophilic microorganisms (e.g., Acidithiobacillus caldus, Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum), and extremely thermophilic microorganisms (e.g., Metallosphaera sedula, Sulfolobus sp., Acidianus sp., and Sulfurcoccus mirabilis). Moderately thermophilic microorganisms have a better prospect in the future industrial application of chalcopyrite bio-hydrometallurgy (Olson, Brierley, & Brierley, 2003; Rohwerder, Gehrke, Kinzler, & Sand, 2003). Hence, investigating the detailed dissolution and passivation mechanisms of chalcopyrite bioleaching by moderately thermophilic microorganisms is of important significance, which will provide essential information for promoting chalcopyrite bioleaching by moderately thermophilic microorganisms in laboratory or industrial plant."