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By Bhavani Kedika, Venkatagiri Noole, Krishna Thotla, Krishna Reddy Chepyala

Cationic lipids are attractive candidates for transgene delivery and expression and are safer alternatives to viral delivery vehicles. Here, we report the design, synthesis, characterization and transgene expression efficacies of a series of chrysin derived cationic lipids. A series of ten lipids of chrysin was synthesized by treating the 7-hydroxy group with dibromoalkane and further substitution of the bromo derivative with alkyl amines. The synthesized lipids were characterized spectroscopically. Parallel screening of the synthesized lipids for in vitro plasmid delivery expressing luciferase led to identification of several leads that demonstrated higher transgene expression when compared to the standard. Transgene expression efficacy was dependent on the chain length of the lipid. The influence of the various physicochemical properties on transgene expression efficacies was investigated. This study indicates that lipids derived from chrysin results in high levels of transgene expression and may be attractive for gene therapy applications.

Mar 1, 2017

Cospidie (3CaO*2SiO2*CaFe2) is one of the most important compounds crystallized in mold flux films during casting in the continuous casting process of steel, The primary crystallization field of cuspidine has been experimentally developed in the CaO-SiO2-CaF2-SiF4 system. The cuspidine-NaF binary and cuspidine-CaF2-NaF ternary systems have been also experimentally determined to study effects of addition of NaF on the primary field of cuspidine. In order to discuss its thermodynamic stability the standard Gibbs energy of formation of cuspidine has been deterimed by an electromotive force methods using CaF2 as solid electrolyte and a transpiration method as follows: ^fG° (cuspidine) /kjmol-1 = 5198 + 0.825T (+12) (1313<T/K<1329): EMF method ^fG° (cuspidine) /kjmol-1 = 5178 + 0.813T (+8) (1221<T/K<1469): Transpiration method The chemical potential diagram and constituent activities for the CaO-SiO2-CaF2 System have been evaluated based on the obtained thermodynamic data.

Jan 1, 2003

By Ramana G. Reddy, Yudong Li

Numerical modeling of the thermal plasma process was carried out based on the thermal plasma reactor in our lab and confirmed using experimental data. The inlet boundary conditions of a non-transferred DC arc thermal plasma reactor were used in modeling the temperature and fluid flow distribution in the reactor. Different mesh grid sizes were used to confirm the model is independent of grid size. Temperature profile and gas flow distribution in the thermal plasma reactor were developed by the computational fluid dynamics (CFD) with ANSYS Fluent. The predicted temperatures are in good agreement with the experimentally measured temperatures in the reactor. The influence of plasma torch input power as well as the plasma gas flux on the temperature distribution was investigated using this model. The influence of power input and gas flow rate on temperature and velocity distributions are not independent. Generally, higher power input and lower gas flow rate will give rise to the temperature increase in the reactor.

Mar 1, 2017

By S. Fitzel

The Betts process for electrorefining of lead has been in operation since 1902 in the Trail Operations Metallurgical complex. The Betts process allows production of high purity lead in a single electrochemical step. Process improvements and plant expansions have taken place over the many decades of operation of this process which has proven to be robust, flexible, and capable of successfully treating a large range of anode bullion compositions. This paper reviews the history of the Betts lead electrorefining process, the innovations and quick industrial adoption of new discoveries that took place at the end of the nineteenth century. The process was first implemented in Trail Operations and integrated very well with metallurgical needs then and now.

By Tsembel Darjaa, Toru H. Okabe, Yoshiaki Umetsu

"With the purpose of extracting molybdenum and rhenium from molybdenum sulfide concentrate; .electrolysis of slurry, solvent extraction and ·precipitation operations were carried out. The slurry of the concentrate in 10 mass% NaCl aqueous solution was continuously circulated into a vertical type 10 stack bipolar electrolytic cell consisted of carbon electrode disks, and, the concentrate was dissolved by electrochemically generated oxidizers at 313 K. The current efficiency for molybdenum dissolution was 73 % after 10 hours electrolysis, and the leach solution was found to contain 0.2 mol/l of molybdenum and 4x10-5 mol/l rhenium. This result shows the dissolution is more than 8 times faster than that obtained using a single cell. The leach solution from electro-oxidation was then subjected to a solvent extraction for rhenium which is then recovered as sulfides. After rhenium separation, molybdenum was precipitated by pH adjustment, and solid MoO3 (H2O)x crystals, free of sulfate, were obtained from the leach solution containing large amount of so42-.IntroductionIn the past, electro-oxidation using a bipolar cell was applied for leaching molybdenite concentrate and ores at U. S. Bureau of Mines [1-3] and also at Bhabha Atomic Research Center [4]. In· these previous studies, Moebious type bipolar cells, in which electrode plates were vertically inserted into the free surface of the electrolyte, were employed and its effectiveness was demonstrated.Recently, the present authors studied on leaching of Mongolian molybdenite concentrate, and the effectiveness of leaching of the concentrate by electro-oxidation in alkaline and acidic NaCl solutions was demonstrated [5]. The leaching reaction during the process can be explained by the 18 electron transfer reaction of molybdenite (MoS2) with the oxidizers produced in-situ by electrolysis of NaCl aqueous solution. Further investigation was made with respect to various process parameters for electrooxidation such as pH, pulp density and temperature of the slurry, and the dissolution behavior of minor elements such as iron, copper, and rhenium [6]. Solubilities of molybdenum in the leach solution at, various temperatures and pH .was · studied, and feasibility of molybdenum recovery by hydrolysis of molybdenum species was demonstrated. By adjusting the pH of the leach solution, molybdenum oxides or hydroxides free from sulfate were successfully obtained from the solution containing large amount of sulfate ion. Rhenium present in the concentrate at a level of several tens of mass ppm was found to dissolve into the leach solution, and co-precipitate with the molybdenum compounds. Rhenium in the leach solutions, therefore, has to be recovered before precipitation of molybdenum, otherwise it is lost in the precipitate."

Jan 1, 2000

By Patrick R. Taylor

In the search for the development of more "environmentally friendly" and more intensive waste disposal approaches to meet more and more stringent environmental regulations, thermal plasma-based processes? are considered one of the most promising alternatives to well-established technologies such as incineration. This paper presents an overview on the research and development status of thermal plasma-based waste remediation technologies. The properties of thermal plasma are described. Due to its unique feature of high-energy intensity and super high temperature, thermal plasma is particularly capable of destroying organic-containing wastes. Its potential and promising application to waste remediation may include: treatment of incinerator ashes; metallurgical dusts (especially, electric arc furnace dust); medical wastes; radioactive wastes; and other Resource Conservation and Recovery Act (RCRA) listed hazardous wastes. Many research institutes and vendors have been involved in the development of thermal plasma-based waste treatment-technology encouraged by the cleanup programs supported by the Environmental Protection Agency (EPA), the Department of Energy (DoE) and the Department of Defense (000). More recently, thermal plasma-based waste treatment technologies (sometimes called plasma-enhanced pyrolysis) are focused more and more on not only just destroying organic wastes but also on how to practically, and economically recover energy contained in the organic waste materials in addition to the recovery of metals in order to offset the high operating costs.

Jan 1, 2003

By J. T. Stanley

The identification of crystal phases and crystal chemistry can be critical to understanding the mechanisms of waste material interaction with native soils. Understanding these mechanisms can suggest correct methods for remediation and treatment. A program is outlined and examples are given of the development of effective techniques for rapid phase identification of environmental samples using electron microscopy. The program concentrates on the development of integrated object oriented software to aid rapid searching of online databases and rapid simulation of potential crystal diffraction patterns. Examples come from dried sludge taken from a former hard-chrome plating facility.

Jan 1, 1993

By Walter Curlook

An improved process of hydrometallurgical treatment of laterite ores of the limonitic type for the recovery of nickel and cobalt using sulphuric acid has been developed and patented [I]. The major improvement is brought about by recycling a significant portion of the "mother liquor" emanating from the pressure leaching reaction back to the feed preparation stage thereby substituting for all or at least a major proportion of the fresh water that must be added. The amount of process effluent waters is correspondingly reduced. Concomitantly with the major savings in fresh water requirements, a degree of reduction in new sulphuric acid requirements is effected, along with a savings in limestone and lime required for subsequent neutralizations. Keywords: Acid pressure leaching; Nickel laterites

Jan 1, 2004

By S. Agatzini-Leonardou

The objective of this work was to prove repeated loading capacities and investigate the selectivity of physically stabilized algal biomass from Spirulina platensis and Chlorella sp. for the removal and recovery of cobalt, nickel and chromium from laterite heap leach liquors. The testwork was carried out on a bench scale in small glass columns and proved that it was possible to immobilize the biomass on the highly porous surface of pumice stone (kissiris). Nickel and cobalt could be removed from pure aqueous solutions of each metal by the immobilized cyanobacterial biomass. Nickel and also cobalt biosorption was sensitive to the initial solution pH. Cobalt recoveries up to 80% were achieved after 5 loading cycles, from low concentration solutions, at pH 6.0. Nickel removal was complete under the same conditions, whereas chromium sorption was ineffective. Desorption of the metal loaded columns was effected by dilute sulfuric acid. The algal species giving the best adsorption capacities for the metals under consideration was selected for further experimental work with real laterite heap leach solution produced by column leaching. A statistical analysis of all obtained results was conducted using the fraction of metal removed as response. The ratio "mass of metal removed to mass of protein contained in the immobilized cells" was determined as well. The described method might be applicable for recovery of cobalt and nickel from diluted laterite heap leach solutions, such as heap wash waters, or for their removal from waste streams of a hydrometallurgical extraction process.

Jan 1, 1997

By Thomas E. P. Wechsler

The limiting factor for most pyro-metallurgical processes in secondary cop- per melting is the time required to heat and melt the product. American Combustion&apos;s proprietary PYRETRON variable £uel/air/oxygen combustion system improves the throughput of existing scrap melting and heating furnaces for copper and copper alloy production by increasing the rate of heat transfer from the flame to the product. The increased productivity and energy savings obtained from this technology can result in sizable reductions in operating costs for smelting operations.

Jan 1, 1991

By Jewel A. G. Gomes

Lead has long been an USEPA priority pollutant and an important environmental and health concern because of its toxicity at ppb levels. In light of health and exposure data for lead, the EPA has set standards for Maximum Contaminant Level (MCL) and a MCL Goal (MCLG) as 15 ppb and 0 ppb, respectively. An accurate and rapid measurement of lead in the field remains a technical challenge. In this work, a relatively new method of deploying a nanoband electrode system using anodic stripping voltammetry has been optimized by changing deposition potential, electrolyte, and plating time used for the measurement of Pb(II) in water samples. Green rust has been proven to be an efficient intermediate matrix for removal of lead contaminants. We also report here the removal efficiency of lead using green rust produced by electrocoagulation, both directly and indirectly. Lead containing floc was also characterized through XRD, SEM, and EDAX techniques.

Jan 1, 2008

By Guangliang Wu, Dong-chang Huang, Xin-bin Liu

High temperature mechanical properties of 50Cr5MoV roll steel was investigated by Gleeble 1500 thermal simulator testing machine The morphology, composition and microstructure of fracture surface were observed and analyzed by OM, SEM and EDS The results are indicative of that, there are two brittle temperature zones of 50Cr5MoV roll steel at the temperature of 600°C~950°C and 1300°C~1450°C, respectively. The section shrinkage rate is less than 60% The fracture mode changes from mixed ones dominated by intergranular to toughness transgranular one with the increase of temperature at the range of 650°C~1300°C. The fracture is along the solid--liquid phase at the range of 1300°C~ melting point It is crucial to formulate the 50Cr5MoV casting technology, to prevent the cracks and improve the quality of 50Cr5MoV roll steel.

Jan 1, 2016

By F. J. Tavera

Recently Jameson cell technology has gained acceptance in both mineral and non-mineral applications, mainly because of the high degree of contact between the involved phases in the downcomer, fact that leads to an efficient collection performance. The key for an acceptable performance of the downcomer operation depends on the stability of the jet zone, the mixing zone, and the plug flow zone. in this work the behaviour of the downcomer of a laboratory Jameson cell is reported, as a function of gas hold-up, superficial gas velocity, superficial liquid (or slurry) velocity, and length of the operating zones. The presence of carbon is evaluated in terms of its effect on the gas hold-up and bubble surface area flux. The influence of the downcomer operating conditions on the gas-liquid disengaging cell is presented. Keywords: Jameson cell, downcomer, gas hold-up, mixing, plug flow, conductivity.

Jan 1, 2003

By Patrick R. Taylor

In the search for the development of more "environmentally friendly" and more intensive waste disposal approaches to meet more and more stringent environmental regulations, thermal plasma-based processes are considered one of the most promising alternatives to well- established technologies such as incineration. This paper presents an overview on the research and development status of thermal plasma-based waste remediation technologies. The properties of thermal plasma are described. Due to its unique feature of high-energy intensity and super high temperature, thermal plasma is particularly capable of destroying organic- containing wastes. Its potential and promising application to waste remediation may include: treatment of incinerator ashes; metallurgical dusts (especially, electric arc furnace dust); medical wastes; radioactive wastes; and other Resource Conservation and Recovery Act (RCRA) listed hazardous wastes. Many research institutes and vendors have been involved in the development of thermal plasma-based waste treatment technology encouraged by the cleanup programs supported by the Environmental Protection Agency (EPA), the Department of Energy (DoE) and the Department of Defense (DoD). More recently, thermal plasma-based waste treatment technologies (sometimes called plasma-enhanced pyrolysis) are focused more and more on not only just destroying organic wastes but also on how to practically, and economically recover energy contained in the organic waste materials in addition to the recovery of metals in order to offset the high operating costs.

Jan 1, 2003

By Chikabumi Yamauchi

The comprehensive scientific development of conventional industrial technologies stimulates the creation of new ones in the next generation. Prof. Akira Yazawa has just accomplished such an important work in the non-ferrous pyrometallurgical field. The results of his successful research are immortal. The research group of the author has conducted since 1985 fundamental studies on the application of sodium carbonate slag to copper fire refining. In the present paper, a calculation method for the distribution ratios of impurity elements between sodium carbonate slag and molten copper will be shown. The specific treatment by sodium carbonate slag will also be presented as an effective way for reducing each impurity content in the molten copper to 0.1 mass ppm, which is the level required for the production of high purity copper. The thinking-out of this evaluation method gets a hint from the Equation (2) in the text which was proposed by Prof. Akira Yazawa.

Jan 1, 2003

By W. -D. Schneider, B. Schwab

Like all metals, zinc can be recycled theoretically without end. To demonstrate environmentally friendly use of zinc, it is necessary to increase the recycling rate. In this regard, the Imperial Smelting Process developed in the forties and fifties in the United Kingdom has become more and more important. This unique process combined with a sinter plant, hot briquetting, and direct injection through the ISP furnace tuyeres offers ideal possibilities to recycle all kinds of zinc and lead bearing materials. Possible secondary materials include Electric Arc Furnace (EAF) dust, zinc-containing sludges and even zinc/carbon batteries. This high temperature process also destroys all organic materials, like dioxin, and can be seen as a sink for many harmful substances.

Jan 1, 2000

By Stephen J. Hales

State-of-the-art Resistance Spot Welding (RSW) equipment has the potential to permit adaptive feedback through recent advances in computerized process control. The initial step toward achieving this goal involves assessment of the effect of specific variables and the interaction between these process inputs on spot weld characteristics. This investigation addresses this issue through the application of Taguchi methodology to understand and refine a RSW schedule developed for joining of two dissimilar aluminum-lithium (AI-Li) alloys of different nominal thickness. The experiment was designed to establish a hierarchy of inputs with respect to each &apos;other, and to determine the significance of these inputs with respect to experimental noise. Valuable insight was gained regarding the interaction between process variables and the effect of certain equipment-related changes. In spite of a decrease in equipment performance, an improvement in the maximum strength attainable for defect-free spot welds resulted from the application of the statistical experiment.

Jan 1, 1992

By Melinda Channel, Alan W. Weimer, Jonathan R. Scheffe

"There is a need to develop cost effective renewable energy processes for the production of clean fuels that can simultaneously mitigate the U.S. energy/global environmental security issue. If H2 can be obtained cost effectively via the splitting of water using concentrated sunlight, then it’s possible to operate a fuel cell using renewable H2 or to synthesize other fuels based on renewable H2 for generating electricity or for running internal combustion engines. For example, H2 can be reacted with sequestered carbon dioxide (CO2) to produce syngas (H2/CO) via reverse water gas shift (RWGS), i.e. H2 + CO2 ? CO + H2O. The syngas can be catalytically methanated to form synthetic natural gas (SNG) or it can be catalytically reformed to produce liquid fuels such as “green” gasoline via the MTG process (syngas? methanol ? gasoline). The desert SW United States is home to world class sunlight. Fundamental ferrite thermodynamics will be reviewed, an example of the beneficial effect of ferrite surface area on reaction kinetics will shown, and process economics will be investigated for a 100,000 kg/day facility to produce H2.Introduction and Background ThermodynamicsSpinel ferrites of the form MxFe3-xO4, where M generally represents Ni, Zn, Co, Mn, or other transition metals, have been shown to be capable of splitting water according to the two step cycle shown below:MxFe3-xO4 + solar thermal energy ? xMO + (3-x)FeO + 0.5 O2 (1)xMO + (3-x)FeO + H2O ? MxFe3-xO4 + 2H2 (2)This is an inherently clean and sustainable process, as the only net inputs are solar energy and water, and the net outputs are hydrogen and oxygen. Ferrite water splitting cycles are advantages compared to other high temperature water splitting cycles, such as the ZnO/Zn and Mn2O3/MnO cycles, because thermal reduction has been demonstrated at significantly lower temperatures[1, 2]. As a result, overall efficiency may be increased due to the fact that radiation losses increase as the fourth power of temperature, and material considerations for reactor design should be more flexible. Additionally, cycle repeatability has been demonstrated with relative ease as compared to other thermochemical cycles [3-6]. However, the amount of hydrogen per mole that is generated is significantly less than for other high temperature thermochemical cycles due to the fact that only Fe3+ is capable of being reduced at the temperatures of interest[6]. In addition, water oxidation kinetics appear to be very slow, likely due to diffusion or surface area limitations created by near surface oxidation [6, 7]."

Jan 1, 2010

By John Snowberger III, J. Brent Hiskey

The electrochemical dissolution of super alloys containing different amounts of rhenium was investigated. The initial part of the investigation focused on the anodic dissolution of rhenium from second-generation (3% Re) and third-generation (6% Re) nickel based alloys. Exploratory electrochemical tests were conducted to evaluate solution chemistries and to establish fundamental dissolution behavior of the alloys. These included rotating disc electrode (RDE) cyclic voltammetry, linear sweep voltammetry, and chronoamperometry tests. Electrolytes comprising sulfuric acid, hydrochloric acid, and ammonium sulfate were examined. Of these, a 0.1 M HCl solution proved to be extremely effective for electrochemical dissolution of samples of CMSX-4, CMSX-10, and SC-180 alloys. This paper discusses in detail the electrochemical behavior of these alloys.

Jan 1, 2014

By Hong Ouyang

A unified computational model has been developed for the Bridgman growth processes of B-NiAI and CdTe crystals. To handle the geometrical and physical complexities, a two-level approach has been developed. At the gtobatlevel, conjugate heat transfer, phase change dynamics, and variable material properties along with the realistic geometries and thermal boundary conditions are accounted for inside the entire crystal growth system. Refined calculations are then made within the ampoule, with the boundary conditions supplied by the global simulations. This model can optimize the use of computational source, help understand solidification behaviors, and improve designs of crystal growth system.

Jan 1, 1996