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By Z. Y. Lan

Bioleaching of marmatite with various mixed cultures of microorganisms was investigated. The experiments were carried out in shaking flasks with iron-free nutrient and the pulp density of 10% wt/vol at the temperature of 30?. The results show that zinc is preferentially leached before iron. The leaching rate of zinc with the mixed culture of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Leptospirillum ferrooxidans is 62% in 18 days. The leaching rate of zinc with the mixed culture of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans is nearly the same as that with the mixed culture of Acidithiobacillus ferrooxidans and Leptospirillum ferrooxidans (45% versus 50%). SEM and EDX analysis of the leaching residues indicate that a solid product layer composed of iron sulfide, elemental sulfur and jarosite accumulates in the mineral surface and becomes a barrier to the dissolution of marmatite. The bioleaching of marmatite follows the shrinking core product layer model. When the product layer increases and becomes insoluble, the leaching rate is controlled by the diffusion through the product layer and slows down. When the product layer decreases or becomes a porous layer, the leaching rate is controlled by chemical reaction and enhanced dramatically.

Jan 1, 2014

By Morin D. H., Battaglia-Brunet F., D'Hugues P.

"In the mineral-processing field, bioleaching of metal sulphide concentrates is currently operated in very large mechanically agitated tanks. Such bioreactors have considerable requirements in terms of power consumption for mixing, aeration and heat transfer. The use of slurry bubble column, where the particles are suspended by gas motion as well as by liquid upward flow, could be an alternative to achieve bioleaching in more economical conditions. In order to compare these two techniques, a unit of stirred reactors in cascade and a bubble column were comparatively operated at laboratory scale in the bioleaching of a pyrite concentrate. For both pieces of equipment, experiments were performed not only in batch mode but also in continuous conditions.Bubble column performances were significantly affected by unpredictable technical difficulties met to maintain continuous feeding in the bubble column and by a significant settling of the heavy solid particles. Nonetheless, theoretical kinetic data, extrapolated from results in batch conditions, could be compared to those obtained in continuous tests, in bubble column and in mechanically agitated reactors. These kinetic data were used to evaluate the influence of reactor design and configuration choice on bioleaching performances."

Jan 1, 2003

By M. Oliazadeh

The bioleaching of fine low grade copper sulfide ore is described. A sample of ground (> 1 mm) copper ore from Sarcheshmeh, Iran, was subjected to a series of agglomeration procedures and subsequently leached. The best agglomerate was obtained by using lime and sulfuric acid as binders. A column bioleaching test of agglomerated ore was then run for 105 days along with a non-inoculated control column having thymol as bactericide. The results show that about 77.5% of copper was extracted in the bioleaching, whereas the control column achieved only 57% copper leaching.

Jan 1, 2007

By D. L. Lampshire

Biological leaching using native heterotrophic bacteria was studied by the U.S. Bureau of Mines as a means of extracting manganese from a domestic low-grade wad ore. Column heap leaching simulation techniques were employed using molasses as the nutrient source for the bacteria. These column studies were designed to investigate the effects of molasses medium flow rate, concentration, and replacement interval on the extent and rate of manganese extraction. .Results showed that flow rates between 0.41 and 8.1 L/min/m2 had little impact on manganese extraction. The total amount of molasses used did have a direct effect on the extent and rate of manganese extraction. Manganese extractions over 90-pct were obtained in 12 weeks of bioleaching with 2-week medium replacement cycles.

Jan 1, 1993

By D. J. Adams

Cyanide heap leaching is the predominant technology used in processing low-grade gold ores. During closure ora heap leach operation, residual cyanide must be removed from the process and waste solutions, as well as the heap. Biological cyanide oxidation is a proven, economical technology for destroying cyanide in process and waste waters and spent heaps; The use of biological cyanide degradation eliminates the need for toxic or corrosive chemical oxidizers and has- been implemented at full scale at treatment costs usually <$0.50/1,000 gal. The Applied Biosciences biological cyanide destruction (BCND?) process has been demonstrated to effectively treat cyanide, concentrations up to 350 mg/L. Treated process solutions and wastewaters also contained other contaminants such as arsenic, copper, iron, silver. selenium, mercury, nitrate and zinc, much of which was removed in the cyanide degradation process. Under optimal conditions, microorganisms can rapidly oxidize free cyanide in some process solutions from over 250 mg/L down to 0.1 mg/L in 4 to 5 hr. Cyanide is degraded to ammonia and carbon dioxide, with 50%ofthe cyanide carbon liberated as C02. In general, carbon tank based bioreactors degrade cyanide at significantly higher rates than process or wastewater pond configured treatments, which usually degrade cyanide-more rapidly than in heap treatments. Investigation of cell-free enzyme preparations as an alternative to live microbial cyanide degradation shows promise. Advantages of enzymes over live microbes for cyanide degradation include: (I) the ability to tolerate and degrade higher cyanide concentrations (> I ;000 mg/L), (2) nutrients to support live microbial cells are not required, (3) the effects of other toxic contaminants, such as metals, found in mining waters-are eliminated and (4) the potential for greatly increased kinetics.

Jan 1, 1998

By I R. F MacCulloch

Bacterial oxidation of sulfide minerals is a familiar and commercially available process to enhance gold recovery with problem ores. Pintail Systems, a Colorado, USA based company had developed bio-processes for gold recovery based on natural mineral formation and transformation in a global mineral cycle. The biological recovery of precious metals from ore involves a number of mechanisms that are dependent on the metal-mineralogical association. These processes include: partial bio-oxidation of sulfide ores; oxidation of gangue minerals exposing gold to leaching; surfactant properties of bacteria/nutrient solutions that improve wettability of ores and solution contact with leachable gold and silver; and microbial production and excretion of trace amounts of gold lixiviants, and bio-fracturing or biological alteration of ore minerals. Pintail has demonstrated enhanced recovery of precious metals during cyanide detoxification of spent heaps at the end of mine life. Biological recovery processes also have the potential to be applied to in situ mining technologies. The in situ process application could recover gold from subgrade deposits, small deposits or those ore bodies with uneconomic stripping ratios for conventional mining. In situ biomining can be accomplished at a fraction of the cost of conventional mining and leaching and does not have the environmental liabilities associated with many conventional gold lixiviants.

Jan 1, 2004

By J. D. Isbister

Coal is the most abundant and most economical source of energy in the United States, however, combustion of coal results in release of pollutants to the environment. The release of sulfur dioxide and oxides of nitrogen during combustion of coal contributes to the deterioration of the environment. Energy independence in the United States must include the increased use of coal reserves. Increased reliance on coal for energy will dictate the necessity for making coal a cleaner fuel. New technologies employing a variety of techniques from harsh chemical treatment to mild physical separations to clean coal are rapidly becoming available. Success for any coal cleaning process is in economics which translate into efficient removal of sulfur with high recovery of combustibles as well as low capital and operating costs for the process. Conventional coal cleaning processes remove water soluble sulfates and a portion of the pyritic sulfur and ash from coals. Removal of additional sulfur from coals requires more advanced coal cleaning techniques. Coal contains many organic sulfur forms which are the most difficult of the sulfur types to remove from coal. The heteroaromatic sulfur species, such as the comlex thiophenes, are the most commonly found organic sulfur forms. These sulfur forms are resistant to chemical attack and are considered to be refractory compounds. Atlantic Research Corporation has developed an "unique" microorganism, CB1 (Coal Bug 1), capable of oxidizing thiophenic sulfur in coal to form water soluble sulfates.

Jan 1, 1986

By L B. Sukla, V N. Misra

Microbial desulfurisation of coal has significant advantages over physicochemical desulfurisation processes since the former process selectively removes the inorganic sulfur compounds and heavy metals without significant carbon loss. The purpose of this study was to improve the desulfurisation yield of a coal sample from northeastern coalfields, Assam, India using microbial technique. The coal sample contained 2.13 per cent of total sulfur and 2.59 per cent of ash. Stirred tank reactors of 40 L capacity were operated in batch mode. Initially, the reactor was fed with 40 L of slurry containing ten per cent (w/v) coal, ten per cent (v/v) inoculum of mixed culture of mesophilic acidophiles, native to coal itself and the requisite amounts of constituents for 9K medium without added ferrous sulfate. The percolate of the reactor was then used to seed the medium for the next experiment. The purpose was to obtain a better adapted and more active biomass which would give a better desulfurisation yield for coal. Further studies were conducted in the bioreactor, fed with 40 L of slurry containing ten per cent (w/v) pulp density of coal, ten per cent inoculum (derived from previous studies) and the medium, supplemented with ferrous sulfate. Results showed an increased total desulfurisation yield of 43 per cent in the latter treatment in comparison to the previous one, where the desulfurisation yield was only 23 per cent. The findings also demonstrated the convenience of using inocula derived from coal, where 50 - 60 per cent of pyritic sulfur was removed, while chemical desulfurisation is of little significance with the majority of coal types. The ash content was also reduced to 1.74 per cent as the low pH of the process caused dissolution of a number of common minerals present in the coal.

Jan 1, 2004

By Robert D. Rogers

The demand for phosphate will continue into the future, because phosphate is currently irreplaceable as a plant nutrient and in many chemical applications. Phosphate is not recycled, so the supply must be replenished through a process of mining and purification. Numerous microbial isolates. obtained from the environment were screened find those which demonstrated the greatest promise of solubilizing PO4 from the ore of interest. Screening was accomplished in a step wise manner an agar plate bioassay, shake flask studies, and semi continuous growth conditions. It was found that with the proper conditions of nutrient supply and solution pH; the bacteria and. fungus used in the semicontinuous process were able to solubilize in excess of 80% of an Idaho rock phosphate ore (RP) within nine days. A significant amount of solubilization occurred within the first six days. There was a &apos;reasonable correlation between the occurrence of soluble Ca and PO.4 during the processing of the RP thus providing evidence that the RP was being disintegrated by the organisms. In all cases, HPLC analysis of the solutions in which PO4 had been. extracted from RP showed that organic acids, which have been implicated as the mechanism of solubilization, were present.

Jan 1, 1991

By Daniel C. McLean

Extraction data for Cu, Zn and Fe were obtained from 4.5" and 10" columns using massive sulfide ore and natural acid mine water. The purpose was to determine if recycling of solution through the column would produce leach liquors of 4 g/l Cu and Zn at extraction rates of 1 g/l per pass at typical flow rates of 0.002 to 0.006 gpm/sq.ft. Test results exceeded expectations. Cu and Zn concentrations of 4 g/l were obtrained within 4 to 6 cycles. Concentrations greater than 6 g/l Cu and 8 g/l Zn were reached within 10 cycles. Concentrations of greater than 40 g/l of Fe and free sulfuric acid were obtained with no decrease in biological activity being observed. No reagents were used.

Jan 1, 1995

By K. A. Natarajan, S. Usha Padukone

"An industrial waste liquor having high sulfate concentrations was subjected to biological treatment using the sulfate-reducing bacteria (SRB) Desulfovibrio desulfuricans. Toxicity levels of different sulfate, cobalt and nickel concentrations toward growth of the SRB with respect to biological sulfate reduction kinetics was initially established. Optimum sulfate concentration to promote SRB growth amounted to 0.8 - 1 g/L. The strain of D. desulfuricans used in this study initially tolerated up to 4 -5 g/L of sulfate or 50 mg/L of cobalt and nickel, while its tolerance could be further enhanced through adaptation by serial subculturing in the presence of increasing concentrations of sulfate, cobalt and nickel. From the waste liquor, more than 70% of sulfate and 95% of cobalt and nickel could be precipitated as sulfides, using a preadapted strain of D. desulfuricans. Probable mechanisms involving biological sulfide precipitation and metal adsorption onto precipitates and bacterial cells are discussed.IntroductionIndustrial effluents from hydrometallurgical and mineral processing operations often contain high sulfate and metal concentrations posing significant disposal problems, which require urgent solution to avoid serious environmental contamination. In such waste liquors, sulfate and heavy metals such as cobalt, nickel, zinc and iron originate from the chemical or biological oxidation of exposed sulfide minerals. Reduction of sulfate and precipitation of toxic metal ions from industrial wastes is essential from the viewpoint of environmentally acceptable waste disposal and recovery of associated strategic metals.Many of these heavy metals are essential for metabolic activity of bacterial cells at low levels. But they exert toxic effects at concentrations encountered in polluted water. Removal of toxic metals through biological reduction of sulfates from industrial waste waters have been practiced for several decades. Metal remediation through common physicochemical techniques such as oxidation-reduction, chemical precipitation, filtration, electrochemical treatment, evaporation, ion exchange and reverse osmosis processes is expensive and not suitable in the case of voluminous effluents containing complexing organic matter and low metal concentrations. Further, strong and contaminating reagents are used for desorption, resulting in toxic sludge and secondary environmental pollution. Biotechnological approaches can be more efficient and cost effective for detoxification of such effluents. Biotreatment/ bioremoval offers several advantages over conventional chemical and physical treatment technologies, especially for diluted and mixed contaminants. Biological detoxification, which involves the use of microbes to detoxify and degrade environmental contaminants, has received increasing attention in recent years (Iwamoto and Nasu, 2001). In addition, valuable metals can be recovered from metal sulfide sludge (Boonstra et al., 1999)."

Jan 1, 2015

By B. Waterman, R. Collins, V. R. K. Paruchuri, P. Gonzalez, H. Dijkman

"INTRODUCTION Mining and processing of sulfide ore bodies has the potential to solubilize sulfate. Gypsum in the host rock, as well as oxidation of sulfides during milling and processing can solubilize sulfate. Acid rock drainage (ARD) also has the potential to generate waters high in sulfate. Even after treatment of the ARD with lime, the treated water can have sulfate present in gypsum saturation levels. Most mine-sites reuse sulfate impacted water but as a mine nears closure, reuse options may be limited and water treatment becomes an option. Furthermore, sulfate discharge requirements, particularly for anti-degredation narrative standards, can be very strict. Freeport has investigated many new technologies to economically remove sulfate from these types of water. A Dutch company, Paques B.V. developed one of these technologies and it utilized biological reactors to convert sulfate into elemental sulfur in a 2-step process. In 1992 the first industrial reference of the SULFATEQTM technology was commissioned at the Nystar Budel zinc refinery in the Netherlands. PILOT PLANT TECHNOLOGY AND DESCRIPTION As shown in Figure 1, the plant contains several process steps, which can mainly be divided into the following sections: 1. Anaerobic biological system in which sulfate is reduced to hydrogen sulfide by sulfate reducing bacteria. 2. Aerobic biological system in which hydrogen sulfide is oxidized to elemental sulfur by sulfide oxidizing bacteria. 3. Solid/liquid separation in which sulfur, calcium carbonate and biomass solids are separated and dewatered. Sulfate impacted ground water is pumped to the feed water tank through a plate and frame heat exchanger to maintain the solution temperature around 95°F. A custom mix of micronutrients is dosed to the feed tank. This nutrient mixture provides essential elements for the growth of anaerobic and aerobic biomass. Urea is added to the feed tank and provides the required nitrogen source to the biomasses. The pretreated feed water is pumped into the anaerobic reactor. The reactor works on the gas lift principle. Sulfate reducing bacteria utilize hydrogen gas as electron donors and reduce sulfate to hydrogen sulfide. This reaction increases the alkalinity of the solution. Carbon dioxide gas is added in order to control the pH within working range of the reactor. Due to the low solubility of the hydrogen gas, the anaerobic gas mixture is recycled through the system. The gas mixture recycle also provides the gas lift for mixing within the anaerobic reactor."

Jan 1, 2016

Minerals processing operations use a significant quantity of fossil carbon to provide energy and as reductant. Greenhouse gas emissions from the use of this fossil carbon contribute to the global increase in anthropogenic CO2 emissions to the atmosphere. In the spirit of fostering thinking about a step change in this situation, this paper explores opportunities for the use of sustainable carbon from biomass sources in a range of mineral processing operations. The exploration shows that many common processes are contenders for fossil carbon replacement and that feasible scientific research by minerals processing technologists would add to deeper understanding of the potential for a sustainable bio-carbon based industry. It is also shown that there are many dilemmas on the social, environmental and economic fronts to be solved before a biomass based industry could become a reality.

Jan 1, 2004

By E. T. Pecina

Bioleaching is a viable option for the processing of auriargentiferous refractory ores with high arsenic content. The application of mesophilic (A. ferrooxidans and ?L. ferrooxidans?) and thermophilic bacteria of the genus Sulfolobus (S. solfataricus and S. acidocaldarius) as oxidant treatment before the cyanidation of an industrial arsenical pyrite concentrate is presented. The results show that extraction of gold and silver increases due to bio-oxidation caused by mesophilic bacteria and results in a maximum gold recovery of 68%. In all strains studied, biooxidation resulted in an average silver recovery of 50%. The results showed that all evaluated strains were sensitive to solids content (5 to 20%) and average particle size (75 and 38 µm) of the concentrate.

Jan 1, 2010

By S. K. Palm

The commercial life of a mineral product can be long, but like hitech electronic and software products that soar in sales one day to become obsolete and forgotten a few months or years, mineral products have a life cycle. They are invented, commercialized and eventually decline and disappear from commerce. Diatomite and perlite are produced from mined minerals, but almost none of the products in these industries spring from the earth in finished form. Straight calcined diatomite was invented over 100 years ago and flux calcined diatomite just a few years later. The expansion properties of perlite were discovered and first exploited about 75 years ago. Some applications for diatomite have disappeared or currently face competition from newer products. Dry cleaning was once the second-largest application for diatomite but disappeared over 30 years ago. Both the beer and wine filtration markets for diatomite face increased competition from membrane filters. A pipeline of new, proprietary products is critical to the future of the industry.

Mar 2, 2022

By Ross W. Smith

Microorganisms are increasingly finding use in mineral processing and hydrometallurgy both for the enhancement of mineral engineering operations and for the remediation of mineral industry wastes. Some of the applications, such as biologically assisted leaching of sulfide ores and biooxidation of refractory sulfide gold ores, are established commercial processes. Others, such as the use of organisms for the removal of heavy metal ions from dilute aqueous streams, are nearing commercial application. Other uses of microorganisms are potentially possible. These include use of microorganisms in leaching non-sulfide ores, the flocculation or flotation of minerals and remediation of toxic chemicals discharged from mineral engineering operations.

Jan 1, 1993

By William A. Apel

Several different strains of bacteria have been shown capable of reducing hexavalent chromium, Cr(VI), to Cr(III), a less toxic, .. more readily recoverable form of Cr. This paper examined the Cr(VI) reduction efficacies of 3 different Cr(VI) reducing bacteria: Pseudomonas f1uorescens LB300., Pseudomonas aeruginosa PAOI, and Pseudomonas aeruginosa PAOI (pCROI) It was demonstrated that all three bacterial stratns reduced Cr(VI) primarily during their exponential growth phases while using glucose as their carbon and energy source. Significant differences in the three organisms&apos; Cr(Vl) reduction capabilities depending on initial Cr(VI) concentrations were also demonstrated. P. aeruginosa PAOI showed superior Cr(VI) reducing capabilities over the. entire range of Cr(VI) concentrations tested while the Cr(VI) reduction capabilities of the other two strains were increasingly inhibited as Cr(VI) concentrations increased.

Jan 1, 1991

By Melitza Cornejo

La lixiviación con cianuro es el proceso utilizado por la industria minera para extraer oro y plata. El cianuro es un compuesto tóxico para la mayoría de los organismos vivos. Sin embargo, muchos microorganismos son capaces de tolerarlo e incluso degradarlo. En ese sentido, el texto caracteriza y analiza la composición de las comunidades microbianas de suelos y aguas contaminadas por residuos de cianuro, producto de la actividad minera en la región La Libertad a través de la metagenómica dirigida al ADNr. De forma que se logró aislar e identificar molecularmente 89 cepas microbianas, como los géneros Pseudomonas, Bacillus y Alcaligenes. Asimismo, la capacidad de degradación del cianuro ha sido establecida en base a pruebas in vitro considerando estas cepas, ya sea individualmente o en consorcios.

Jan 1, 2017

By B. Y. M. Bueno

Biosorption of heavy metals can be an effective process for the removal of heavy metals ions from aqueous solutions. In this study, the adsorption properties of Rhodococcus opacus biomass for Pb(II) and Cu(II) ions was investigated. The effects of initial pH, initial metal concentration and biomass concentration on biosorption efficiency were studied in a batch system. Results showed that the initial pH would play an important role in the Pb(II) and Cu(II) ions removal by R. opacus and affected the zeta potential of R. opacus. The equilibrium adsorption was reached with 60 min. The Langmuir and Freundlich models were used for describing of adsorption isotherm data. The maxi-mum adsorption capacities of biomass were determined as 94.3mgg-1 and 32.2 mgg-1 for the Pb(II) and Cu(II) ions, respectively. The experimental data fitted to pseudo second order kinetic model. Adsorption capacity of Pb(II) ions was found to be reduced by the presence of the other competing metal ions in the system. The FT-IR results of R. opacus biomass showed that biomass has different functional groups and these groups are able to react with metal ions in aqueous solution.

Jan 1, 2014

By Javier Enrique Basurco Cayllahua

In this work, a gram-positive bacteria was used as biosorbent to elucidate the aluminum load capacity under different conditions related to metallurgical and chemical plants. The sorption data followed the Langmuir, Freundlich, Dubinin-Radushkevich isotherms. In order to determine the best isotherm fit, three error analysis methods were used to assess the data: correlation coefficient (R2), residual root mean square error (RMSE) and Chi-square test. The error analysis established that the Dubinin-Radushkevich model fits better the aluminum biosorption data. The maximum sorption capacity was found to be 41.59 mg.g-1. The maximum removal of aluminum was 95% at pH around 5. The experimental kinetics data was evaluated considering two models (pseudo-second order and pseudo-first order). This study indicated that the R. opacus is an environmental friendly biosorbent.

Jan 1, 2009