Search Documents

By C. Ringas, F. P. A. Robinson

Various theories proposed to account for microbial corrosion are reviewed as a background to a better understanding of this type of corrosion in iron-based alloys. Experimental evidence is presented to show that even stainless-steel alloys are prone to microbial attack by sulphate-reducing bacteria. The corrosive attack by the bacteria is due primarily to the hydrogen sulphide that is produced as part of their metabolic processes.

Jan 1, 1987

By Chandra Sekhar Gahan

The present study aimed to investigate the depyritization of coal using iron oxidizing and sulphur oxidizing acidophilic chemolithotrophic microorganisms in an iron free 9K growth medium at varying pulp densities. Three different coal samples used in the shake flask study from three different countries such as domestic anthracite (Korean), Indonesian and Chinese lignite. Experiments were carried out in batch at a controlled temperature of 35 °C and varied pulp densities of 8%, 10% and 12% (w/v) with a working volume of 100 ml containing 10 ml of microbial inoculum and 90 ml of the iron free 9K growth medium. Coal samples were crushed and ground to fine size fractions <100 microns by attrition mill wet grinding. The progress in the experiments were followed by the regular measurements of pH, redox potential, viable planktonic cell count, residual Fe2+ ion concentration, amount of acid addition and amount of slaked lime addition. The pyritic sulfur removal percentage varied between 90-95% with highest sulfur removal with China coal (95%) followed by Korean Coal (93-94%) and slightly lower level for Indonesia coal (90%). However the pyrite oxidation was highest for Korean coal ranging between 78-80% followed by a similar level of pyrite oxidation of 50-61% for the Chinese and Indonesian coal. It was found that 10-12% pulp density would be fairly feasible for all the different coals tested leading to an economical process. Another benefit from this study is the iron free 9K growth medium, which will reduce the operation cost and overall it?s an environment friendly process.

Sep 1, 2012

By J. Murphy

The economics of microbial coal desulfurization are affected significantly by the proper selection of the plant site and by maintenance of variables at suitable levels. High sulfur coal particles below 200 mesh show sulfate production rates triple those with 150 mesh coal but grinding costs are greater. Slurry solids density increases from 5% to 20% reduced the rate constant by a factor of 10. Coal added to a batch reactor in the stationary phase resulted in a sudden second exponential sulfate production phase indicating feasibility of lower cost continuous operation. Successful operation in the absence of beef broth, yeast or added nutrients at non-sterile conditions enhanced projected economics. Finally, the need for adequate agitation and aeration was well established.

Jan 1, 1985

By Ashish Kumar

High grade ore deposits in the world are fast depleting and the valuables in the low-grade ores are finely disseminated. The feed grade to flotation circuit is also declining. Attainment of suitable grind is preferred, which inevitably creates more fines of the gangue minerals to interfere with the flotation process because of the undue competition between the fines and the valuables for the collector ion adsorption. Besides the use of polymeric or long chain hydrocarbon depressants for the gangue mineral, microbial induced flotation also has potential to float minerals selectively. Thiobacillus ferroxidans and Thiobacillus thiooxidans adhere on the minerals to modify its surface properties and affect the minerals flotation acting as depressants. Hindustan Zinc has conducted laboratory studies to understand the adhesion of microorganism on the mineral surfaces and its impact on the selective flotation. The experiments were further designed to reduce consumption of depressing reagents for graphitic carbon, pyrite, and galena. Preliminary studies reveal that desired quality of concentrate can be achieved with reasonable recovery. Keywords: thiobacillus thioxidans, thiobacillus ferroxidans, bioflotation, microbial induced flotation

Sep 1, 2012

Coal seam methane (CSM) is an æunconventionalÆ gas resource that is becoming an important energy resource for Australia, especially along the eastern seaboard. It has been conventionally believed that methane in high rank coals was exclusively derived from thermocatalytic processes during decomposition of coal at elevated temperatures. However, recent re-interpretations of gas composition and isotope data have indicated that microbial methane generation may be one of the main processes responsible for the accumulation of commercially producible methane in some coals. In low rank (æbrownÆ and sub-bituminous) coals, methane (CH4) is mainly generated by microbial activity at low temperatures. The amount of this biogenic gas generated and accumulated in low rank coals depends largely on the rate of burial and depth. Most low rank coals are æunder-saturatedÆ with respect to their maximum gas sorption capacity. In contrast, high rank coals may be highly saturated due to extensive gas generation from both thermogenic and secondary biogenic processes. However, in basins where secondary biogenic gases have not replenished the thermogenic gas lost during basin uplift, the coals will be æunder-saturatedÆ. In many basins around the world, high CSM producing zones appear to contain secondary biogenic CH4 generated by microbes transported in meteoric water recharge through permeable coal seams. Biogenic CH4 generation requires the activity of a consortium of anaerobic respiring microorganisms, fermentative bacteria and acetogenic bacteria to break down complex organic molecules to simpler molecules having one or two carbon atoms, which enables methanogenic archaea to generate CH4. The two main methanogenic pathways in the generation of secondary biogenic gas from coal are aceticlastic reaction and carbon dioxide (CO2) reduction. In eastern Australian coals CO2 reduction appears to be the main process. The types of microbes that are directly involved in these processes are unknown because they have not yet been isolated from the coal seams. The CSIRO is currently investigating the processes of biogenic gas generation in Australian coals. Potential may exist to introduce appropriate microbes into deep coal seams to enhance methane saturation levels, gas sorption capacities and permeabilities.

Jan 1, 2004

By Joseph A. Sutton, John D. Corrick

The continuing depletion of high-grade ore de- posits in this country has created a need to develop more effective methods for recovering valuable metals from low-grade ores. The use of microorganisms for their biochemical reactions is one possible way to solve certain phases of this problem. Bacteria have been used successfully by many diversified industries. Until recently, however, little attention has been focused on the use of bacteria in mining and metallurgical processes. This report summarizes one phase of the microbial studies being conducted at the U.S. Bureau of Mines, College Park Metallurgy Research Center. The two-fold objective of this study was, first, to determine if pure strains of the bacteria Ferrobacillus ferrooxidans, Thiobacillus concretivorus and Thiobacillus ferrooxidans could utilize the iron and sulfur occurring in sulfide minerals to produce appreciable quantities of ferric sulfate and sulfuric acid for dissolving copper and, second, to develop the chemistry involved in the microbial oxidation of sulfide minerals. These are of particular interest as they relate directly to the feasibility of employing microorganisms in leaching operations.

Jan 6, 1963

By K. Alibhai

Greek laterites, which generally had nickel concentrations of < 1 %, are amenable to chemical leaching,. although there is considerable variability in the ease of leaching with different ore types. With low grade ores(< 1 % Ni content); >70% of the nickel was leached in columns with a strong selectivity for nickel over iron. Heterotrophic microbially assisted leaching could be a viable alternative to classical hydrometallurgical and/or pyrometallurgical techniques, with a very high recovery (greater than 80% ) being found with some of these mineral microbial interactions. Critic acid was probably the effective chelating agent.

Jan 1, 1991

By John S. Thayer, Frederick E. Brinckman, Kenneth L. Jewett, Gregory J. Olson

Interest and research in biorecovery of metals has focused mainly on cell-based metal leaching and precipitation. However, production of non-enzymatic metabolic products by microorganisms related to metals bioprocessing also deserves attention. For example, it is well known that biogenic inorganic (e. g. H2S04 ) and organic acids play a role in metal dissolution. There are other metabolic products of microorganisms which can catalyze useful metals transformations. These include methylated metabolites such as alkyl halides which can dissolve metals from ores and also reduce certain metal ions in solution to pure metallic particles. This paper describes some of our research on metal dissolution and precipitation by such metabolites. Examples include the production of high purity elemental metal particles of gold, platinum and palladium by volatile metabolic products.

Jan 1, 1989

By G. B. Michaels

Exploration programs for mineral resources often involve analysis of soils or stream sediments for the mineral being sought or its pathfinders. Because many metals are toxic to microorganisms, patterns of metal resistance in soil or aquatic populations may provide additional useful information at a reasonable coat. These patterns in some cases have been more effective than the soil geo-chemistry and may be easier to obtain. Elevated levels of resistance to silver and its path-finders have been demonstrated in stream populations from an area of known silver deposits, and resistance to gold pathfinders has been demonstrated for gold-bearing areas. Uranium has also been located by this method.

Jan 1, 1989

By S. R. Hutchins

Several refractory sulfide and carbonaceous gold ores were examined to determine whether microbial pretreatment could be used to enhance gold recovery. Test samples were biologically leached using unique, thermophilic microorganisms to solubilize iron sulfide minerals. Gold recovery from sulfide ores and concentrates was enhanced following pretreatment with the thermophiles; responses varied widely for the individual materials. Although bioleaching had little effect on the preg robbing characteristics of the carbonaceous gold ores, cyanidation of the bioleached residues yielded higher gold recoveries than cyanidation of the native ore. By combining bioleaching with abiotic processes designed to inhibit preg robbing, gold recoveries of 83-98% were obtained from carbonaceous ores yielding 10-50% gold recovery by direct cyanidation.

Jan 1, 1987

By S. R. Hutchins

Several refractory sulfide and carbonaceous gold ores were examined to determine whether microbial pretreatment could be used to enhance gold recovery. Test samples were biologically leached using unique. thermophilic microorganisms to solubilize iron sulfide minerals. Gold recovery from sulfide ores and concentrates was enhanced following pretreatment with the thermophiles; responses varied widely for the individual materials. Although bioleaching had little effect on the preg-robbing characteristics of the carbonaceous gold ores, cyanidation of the bioleached residues yielded higher gold recoveries than cyanidation of the native ore. By combining bioleaching with abiotic processes designed to inhibit preg robbing, gold recoveries of 83-98% were obtained from carbonaceous ores yielding 10-50% gold recovery by direct cyanidation.

Jan 1, 1987

By J. A. Brierley, S. R. Hutchins, C. L. Brierley

Introduction Many gold and silver ores are refractory to conventional processing technologies. Thus, precious metals recovery is uneconomic. These resources can consist of ores, flotation concentrates, mill tailings, and other reserves. The refractory nature of these materials can be ascribed to such components as iron sulfide minerals, silica minerals, tellurides, organic compounds, and sulfosalts. Precious metals locked within iron sulfide minerals account for a large number of refractory gold ores. These minerals consist principally of pyrite or arsenopyrite. They must be oxidized to liberate the encapsulated precious metal and allow its contact with the leaching agent. Carbonaceous gold ores represent a unique class of refractory precious metal ores in general. Not only does gold encapsulation by iron sulfide minerals occur (Hausen, 1985), but these ores also contain organic matter that interferes with gold recovery by cyanidation. Direct interference can be attributed to either occlusion of gold within organic matter or formation of a stable gold-carbon complex. The magnitude of this problem is debatable. Wells and Mullens (1973) examined two carbonaceous gold ores and found no association between the gold and carbonaceous material. A more common problem is indirect interference, whereby the aurocyanide complex formed during cyanidation is sorbed by the native organic matter and is not available for recovery. This phenomenon is referred to as "preg robbing" (Osseo-Asare et al., 1984; Hausen and Bucknam, 1984). Guay (1981) summarized various methods that can reduce preg robbing during processing of carbonaceous gold ores. However, some are not applicable to carbonaceous ores that have significant amounts of gold locked in iron sulfide minerals. Several methods exist for oxidizing iron sulfide minerals and rendering refractory precious metal ore amenable to conventional recovery processes. These include various embodiments of roasting (Jha and Kramer, 1984; Brown, 1984; Arriagada and Os-seo-Asare, 1984; Smith, 1986), pressure oxidation (Muir et al., 1984; Beattie et al., 1985; Thompson, 1986; Weir et al., 1986; Argall, 1986), and chemical oxidation (Scheiner, 1971; Jackson, 1983; Van Weert et al., 1986). Each process has been successfully used for refractory precious metal ores. In many instances, however, their use is precluded by capital and operating costs relative to the value of the ore. An alternative to these methods is microbial pretreatment - a biological process whereby iron sulfide minerals are degraded and the liberated precious metal values can be recovered by conventional technologies. Bioleaching refractory precious metal ores is a relatively new concept compared to roasting and chemical oxidation. It is rapidly becoming established as a viable pretreatment alternative. Numerous studies have been conducted in the laboratory and on a pilot scale (Yudina et al., 1979; Lawrence and Bruynesteyn, 1983; Pol &apos;kin et al., 1985; Marchant, 1985; Livesey-Goldblatt, 1985; Gibbs et al., 1986). Comparative tests have demonstrated that bioleaching can be equal to or better than roasting or pressure oxidation in terms of product recovery and process economics (Fridman and Savari, 1983; Livesy-Goldblatt et al., 1983; Karavaiko et al., 1985; Gilbert et al., 1986; Hackl et al., 1986). Bioleaching&apos;s main drawback is that pretreatment requires days rather than hours. This can lead to excessive operating costs for difficult-to-treat ores. In all of the tests reported thus far, the leaching microorganism used has been thiobacillus ferro-

Jan 4, 1988

By N. Pradhan, B. K. Mishra, B. D. Nayak, B. K. Mohapatra, R. K. Mohapatra

"Present study is about use of a group of Iron[Fe(III)] Reducing Bacteria (IRB) to convert the goethite (a-FeOOH) present in the original lateritic Nickel ore to magnetite under an anaerobic condition and subsequently release the bound Co(III) and Ni(II) through leaching. The lateritic Nickel ore contains 0.8% Ni, 0.049% Co, 1.92% Cr, 0.32% Mn and 50.2% Fe. An anaerobic dissimilatory Fe(III) reducing bacterial consortium capable of using acetate as carbon source (electron donor) and lateritic ore as terminal electron acceptor, changes the initial light brown color of the ore to dark brown. The change in color is due to the conversion of goethite to magnetite, which was confirmed by XRD. When the IRB treated sample was subjected to both bioleaching and acid leaching, it shows a greater recovery of Nickel and Cobalt values as compared to untreated original lateritic Ni ore. Further a magnetic separation method was used to separate the magnetic part of the treated lateritic ore and subjected to acid- and bio-leaching for better recovery of Nickel and Cobalt.IntroductionIt has been known that micro-organisms have the potential to reduce metals but more recent observations have shown that a diversity of specialist bacteria and archaea can use such activities to conserve energy for growth under anaerobic conditions [12]. Natural Fe(III) oxides are high in surface area and are reactive [1]. Fe (III) oxides adsorb a wide range of metal cations and anions by complexation to surface hydroxyl groups [17], and function as the primary redox buffering solid phase in many sediments and subsurface materials [5].Recently a number of bacteria have been isolated based on their ability to use metal ions, including Fe(III), as electron acceptor under anaerobic conditions. These are capable of producing energy by coupling the oxidation of organic compounds to reduction of Fe(III). Iron[Fe(III)]-Reducing Bacteria (IRB) have been isolated and identified from a wide variety of environments including freshwater and marine aquatic sediments and submerged soil [8]. The biogeochemical cycle of iron is linked to those of the trace metals, as many trace metals coassociate with Fe(III) oxides or reduced iron phases (e.g., magnetite, siderite, or iron sulfide). IRB can utilize Fe(III) oxides as terminal electron acceptors for respiration [18]; thereby reducing all or a fraction of the solid. The reductive portion of the iron biogeochemical cycle in non-phototropic environments (e.g., sediments and subsurface materials) is driven by the direct enzymatic reduction of Fe(III) oxides to Fe(II) by dissimilatory iron reducing bacteria [13,14]."

Jan 1, 2009

By H. Y. Sun, Q. Y. Tan, X. P. Niu, L. Li, Y. Jia, R. M. Ruan

During bioleaching processes of sulfide minerals, the sulfide minerals act as the natural habitats for acidophiles, of which, most are autotrophic iron and sulfur oxidizing microbes. These microbes acquire energy by oxidation of sulfide minerals, and play crucial role in bioleaching processes. In the industrial bioleaching heaps, microbial community and their activity are highly related to engineering configurations, while the optimized industrial operation can help to fast dissolution of aim minerals and acid/iron balance under optimized microbial condition. Microbial community succession and activity formation, the function of microbes to mineral dissolution, the mechanisms to promotion and inhibition of sulfide minerals oxidation, and the industrial instances for microbial community regulation were reviewed. For these instances, promoted microbial activity helped to fast pyrite oxidation for sulfuric acid generation, which realized zero sulfur acid addition to the irrigation solution in the initiation of a bioleaching heaps. High microbial activity by optimized operation parameters improved Cu leaching efficiency during the heap bioleaching process. And also by inhibition of microbial activity, especially the iron oxidizing microbes, the excessive pyrite oxidation can be inhibited, which helped to the acid/iron balance during heap bioleaching. INTRODUCTION Around the world, high grade copper ore are exploited, while barely new high-grade deposits are succeeded, the copper grade of ore deposit is constantly decreasing. The increasing copper demands worldwide requests to process these low-grade ore deposits. Processing of low-grade ores are not economical by usual extraction processes. Heap leaching is a cheaper process for recovery of low-grade ores, and has been applied to extract valuable metals from sulfide minerals worldwide, such as copper, gold, uranium and nickel (Gericke et al., 2009). Heap leaching process has greatly decreased the economic grade and expanded the recoverable ore reserve worldwide (Brierley, 2008). It is usually carried out in the open environments, and could process huge amount of ore (usually in millions of tonnes in one reactor) in the heap reactor (Gericke et al., 2009). For copper sulfide, especially the secondary copper sulfides, heap (dump) bioleaching is successfully applied. Heap bioleaching process is cost saving, and sometimes can be achieved without adding chemical into heaps, by microbial fixing of the atmospheric N2 and CO2, and generating oxidant Fe3+ and sulfuric acid in heaps from oxidation of sulfide minerals. Also heap bioleaching is environment friendly by cycling use of the leaching solution, and without exhaust emission.

Jan 1, 2019

By H. Sarvamangala

Selective separation of alumina, silica, calcite and apatite from hematite was achieved through microbiologically induced flotation and flocculation in presence of bacteria (B.subtilis) and that of hematite from apatite after interaction with yeast (Saccharomyces cerevisiae). Bacterial and yeast metabolites containing extracellular proteins were characterized from mineral-grown cell free extract. Bacteria and yeast can adhere to mineral surfaces and influence subsequent separation of the minerals. Bacteria functioned as a stronger depressant for hematite, while yeast cells depressed apatite at neutral pH. Selective affinity of the bacterial and yeast cells towards the mineral surface was observed through adsorption studies. Extracellular proteins (EP) were isolated from B.subtilis and yeast. The protein profile of the EP of bacterial and yeast cells grown in presence and absence of minerals was studied. This study has demonstrated the utility and amenability of microbially-induced mineral beneficiation of iron ores through bacterial and yeast cells and their metabolic by products.

Sep 1, 2012

By K. A. Natarajan

"Interaction with microorganisms results in significant surface chemical changes on minerals. For example, chemolithotrophs such as Thiobacillus ferrooxidans and Thiobacillus thiooxidans significantly alter the surface chemistry of several sulfide minerals such as pyrite, chalcopyrite, sphalerite and galena. Heterotrophic bacteria such as Bacillus sp. could alter the electrokinetic properties of oxide minerals such as hematite, alumina, silica, kaolinite and calcite. Microbe-mineral interactions could thus be beneficially utilised in mineral beneficiation as different from bioleaching. Microbially induced mineral beneficiation unlike bioleaching deals with interfacial phenomena at mineral-solution-bacteria interfaces and surface chemical changes are brought about at very rapid rates amounting to a few minutes resulting in mineral separations in an aqueous medium.In this paper, the role of acidophilic chemolithotrophs such as Thiobacillus thiooxidans and neutrophilic heterotrophs such as Bacillus polymyxa in the beneficiation of sulfide and oxide minerals is illustrated. Mechanisms of bacterial attachment on various sulfide and oxide minerals are outlined with respect to the influence of bacterial interaction in bringing about mineral surface hydrophobicity or hydrophilicity. The role of bacterial cells as well as metabolic products such as bioproteins and exopolysaccharides in significantly altering the surface chemistry of sulfide minerals such as sphalerite and galena and oxides such as hematite, alumina, silica and calcite is discussed with probable mechanisms. Possibilities of generating mineralspecific bioreagents are outlined with respect to bacterial adaptation to mineral substrates."

Jan 1, 2003

By K. A. Natarajan

Interaction with microorganisms results in significant surface chemical changes on minerals. For example, chemolithotrophs such as Thiobacillus ferrooxidans and Thiobacillus thiooxidans significantly alter the surface chemistry of several sulfide minerals such as pyrite, chalcopyrite, sphalerite and galena. Heterotrophic bacteria such as Bacillus sp. could alter the electrokinetic properties of oxide minerals such as hematite, alumina, silica, kaolinite and calcite. Microbe-mineral interactions could thus be beneficially utilised in mineral beneficiation as different from bioleaching. Microbially induced mineral beneficiation unlike bioleaching deals with interfacial phenomena at mineral-solution-bacteria interfaces and surface chemical changes are brought about at very rapid rates amounting to a few minutes resulting in mineral separations in an aqueous medium.

Jan 1, 2003

By K. A. Natarajan, M. N. Chandraprabha

Selective separation of minerals from complex sulphide ores is a challenging task and it is often difficult to get commercially acceptable individual concentrates by conventional selective flotation using inorganic collector reagents. Microbially-induced mineral beneficiation has proved to be more effective, economically viable and an environmentally benign process for the effective separation of various minerals. Applications of microbially-induced mineral beneficiation are demonstrated in this paper with respect to beneficiation of complex multimetal sulfides. The role of adapted microorganisms and bioreagents in the beneficiation of complex sulphide minerals are also illustrated.

Jan 1, 2009

By M. N. Chandraprabha

This paper discusses the role of the mineral-adapted acidiphilic microorganism Acidithiobacillus ferrooxidans in the beneficiation of arsenopyrite-containing multisulfides (pyrite and chalcopyrite) and the bioremediation of the resulting arsenical waste water. It was found that adaptation to minerals alters the surface properties of the microorganism. Bacterial adaptation to arsenopyrite and controlled bacterial adhesion to mineral surfaces lead to selectivity in arsenopyrite separation. Bioremoval of arsenic ions (both arsenite and arsenate ions) by Acidithiobacillus ferrooxidans is also discussed. Key words: Acidithiobacillus ferrooxidans, Bioremediation, Arsenopyrite beneficiation

Jan 1, 2009

By K. A. Natarajan

The utility of yeast, Saccharomyces cerevisiae, in the separation of quartz from hematite is demonstrated. Yeast cells, as well as their metabolites, functioned as flotation collectors, depressants or flocculants and dispersants for hematite and quartz. Interaction between yeast and the above minerals resulted in significant surface chemical changes, rendering quartz surfaces hydrophobic and hematite hydrophilic. Mineral-specific extracellular proteins and exopolysaccharides were secreted by yeast cells when grown in the presence of quartz and hematite, respectively. Quartz could be efficiently separated from hematite through microbially induced flotation and selective flocculation.

May 1, 2012