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By A. E. Torma

The efficiency of Thiobacillus ferrooxidans in the desulfurization of a high-sulfur content New Mexico coal was demonstrated. The optimum condition of process variables were found to be pH = 2.3, particle size = 38 µm, pulp density 14.1% (W/V) and temperature 33.9°C. Larger scale experiments carried out with these optimum conditions resulted in coal desulfurizations above 97%, while in sterile controls this was only about 10%.- The rate of desulfurization was described with an exponential expression with respect to the effects of coal particle size. The effect of pulp density on coal desulfurization was quantified by a Monod type equation. The apparent activation and deactivation energies, the frequency factor and temperature coefficient were derived from the effects of temperature to be ?aE - 11720 cal mol-1 and A = 30.44 mol dm-3s-lin the range of 20 - 35°C; ?Ed = 43930 cal mol-1 and A = 9.82 x 10-39 mol dm-3s-1 in the range of 35 - 45°C; and Q10 = 1.94 in the range of 20 - 30°C. The thermodynamic values [(?G+, ?H+ and ?S+)] of the activated complex were calculated. The generalized second order regression equation relating the degree of biodesulfurization of coal to the leach parameters was shown to adequately predict coal desulfurization within the range of process variables studied.

Jan 1, 1987

By E. Lee

The behavior of As bioleaching using Acidithiobacillus thiooxidans (A. thiooxidans) in mine tailings containing highly concentrated As contents (i.e., As=33877 mg/kg) was systematically evaluated according to the changes in temperature (25?40°C, pulp density=0.5%, pH=1.8), pulp density (0.5?4%, temp=30°C, pH=1.8), and initial pH(1.8?2.2, temp=30°C, pulp density=0.5%). The changes in temperature and pulp density affected the As leaching behavior more than the change in initial pH did. Based on the pH and oxidation-reduction potential (ORP) results, the As leaching was attributed to solution acidification. In particular, As leaching was accelerated with increasing sulfate production in the reacting solution. After bioleaching, surface of tailings examined that many pores which dissolved As were formed by a chemical reaction due to bacteria. The As bioleaching efficiency by A. thiooxidans was enhanced by the oxidation of both the added elemental sulfur and the sulfur compounds in the mine tailings. The As bioleaching efficiency was maximized at 50.5% under conditions of an initial pH of 2.2, reaction temperature of 30°C, and pulp density of 0.5%.

Feb 23, 2014

By W. M. Zeng

A mixed culture of moderately thermophilic microorganisms was enriched from Acid Mine Drainage (AMD) samples collected from several chalcopyrite mines in China,which can leach copper from chalcopyrite effectively. Its tolerance to chalcopyrite was brought up to 80 g/L after being adapted to gradually increased concentrations of chalcopyrite. The effects of several leaching variables on copper recovery in Stirred Tank Reactor had been investigated. The ore contained chalcopyrite (62.2%) and galena (25.6%) as the main sulfide minerals. The results of the tests show that it is possible, operating at 8% of pulp density to attain copper extraction of 75% in 44 days. The leaching rate of chalcopyrite tends to increase with the dissolution of total iron. Microscopic counts of microorganisms in the solution tend to be higher at low pH range. Furthermore, the analyses of leached residues indicate that the passivation of this chalcopyrite is mainly due to the formation of a mass of jarosite and anglesite (about 48.3% and 21.7% in residue respectively), other than elemental sulphur layer. The bacterial community composition was analyzed by using amplified ribosomal DNA restriction analysis (ARDRA). Two moderately thermophilic bacteria species were identified as Leptospi-rillum ferriphilum and Acidithiobacillus caldus, with different proportion in the bio-pulp, 67% and 33%, respectively.

Jan 1, 2014

By A. E. Torma

In recent years the copper industry has gained considerable economic stability. An essential part of this industrial wealth is attributable to the successful application of bio-mediated heap and dump leaching techniques in the recovery of copper from low-grade resources. This study evaluated the leachability of a chalcopyrite conlentrate using naturally occurring mixed culture of predominantly Thiobacillusferrooxidans and Thiobacillus thiooxidans. For the effects-of pulp density, the overall reaction rate constant (k) and the order of reaction (n) have been determined to be 1.93 h-1 and 1.36, respectively. Cyclic voltammetric measurements revealed that intermediate reactions are involved in the chalcopyrite oxidation. Using the Price approach, it was shown that the dissolution of chalcopyrite is solid state diffusion controlled.

Jan 1, 1992

By J. Vilcáez

The copper extraction yield from thermophilic bioleaching of chalcopyrite depends on temperature, pH, and the oxidation-reduction potential (ORP), as well as the activity of the microbe used. We studied copper extraction yields obtained from chalcopyrite with three thermophiles, Acidianus brierleyi, Sulfolobus metallicus, and Metallosphaera sedula, under various pH and temperature conditions and with different initial amounts of ferric iron. The results indicated that the capacity of each thermophile strain to leach iron as Fe3+ differed, which affected the influence of pH, temperature, ORP, and microbial activity level on the yield of copper. Microbial activity can cause ORP to be close to the critical value (450 mV) at which copper extraction at 65 °C can be higher than that at 80°C. An initial pH of 1.5 retards the precipitation of jarosite, allowing temperatures as high as 80°C to result in copper extraction yields close to 100%. However, if a thermophile with a poor capacity to form Fe3+ is employed, 100% copper extractions can also be obtained at pH 2.5, because ORP is low as result of low amounts of Fe3+ in solution. Optimum temperatures for the growth of thermophiles did not always mean high copper extraction yields. Conversely, high biomass concentrations depending on the capacity of the thermophile strain to leach iron as Fe3+, can result in excess Fe3+ in solution, leading to the precipitation of large amounts of Fe3+ as jarosite. The bioleaching capacity of A. brierleyi was reduced or suppressed when insufficient initial Fe3+ was pro-vided to trigger the leaching reaction, whereas S. metallicus and M. sedula were less sensitive to the initial availability of Fe3+. This result confirmed that a direct enzymatic attack on the mineral surface could initiate the leaching reaction, but later ORP governed the leaching rate of chalcopyrite.

Jan 1, 2014

By V. A. Leão, M. L. M. Rodrigues, L. C. Sicupira, I. C. B. Rodrigues, T. C. Veloso

Brazilian sulphide ores can contain a significant content of chloride and fluoride, which are harmful to bacteria and thus bioleaching. This work summarizes a four-year research investigation on the effect of fluoride on both bacterial growth and sulphide bioleaching, supported by studies carried out with secondary copper ores. Firstly, the influence of fluoride on the kinetics of ferrous iron bio-oxidation with Sulfobacillus thermosulfidooxidans was addressed. Values as low as 10.0 mg/L F- fully inhibited bacterial growth, although fluoride toxicity could be overcome by the presence of aluminium, which reduced the concentration of hydrofluoric acid through the formation of aluminium-fluoride complexes. This required at least a 2:1 aluminium/fluoride molar ratio, which ensured bacterial growth rate values similar to that observed in the absence of fluoride ions. Subsequently, both batch and column bioleaching with mesofiles (30 ºC), moderate thermofile (50 ºC) or hyperthermophiles (67.5 ºC) of two secondary copper ore samples were analyzed with focus on the relevant effects of fluoride-containing minerals on the bioleaching of such sulphide ores.

Jan 1, 2014

By Y. A. Attia

Auriferous sulfide ores often contain very finely disseminated gold and other precious metal particles inside the sulfide crystals. The encapsulation of precious metal particles gives rise to the refractory nature of such ores to current extraction methods. As a result, these fractions are frequently lost in the tailings. The purpose of this work was to investigate the feasibility of pretreating these refractory tailings with bioleaching before employing standard extraction techniques such as cyanidation. Leadville gold ore tailings containing auriferous pyrite were first concentrated by froth flotation. The pyrite concentrate was treated for different periods of time with 12 weeks adapted thiobacillus ferrooxidans before cyanide leaching. The bioleaching of the concentrate before cyanide leaching increased the gold extraction from 32% to 95% and that of silver from 48% to more than 98%.

Jan 1, 1987

By D. Morin

A general study of gold refractory arsenopyrite-pyrite concentrate bioleaching has been carried out including two continuous bioleach testworks at different sizes, namely one at a laboratory scale with a 100 I unit at a 2 kg/d dry solid flowrate and the other at a pilot scale at a 100 kg/d dry solid flowrate. Both tests show a 80% bioleaching of arsenopyrite in about four days of treatment and with the same kinetic. Pyrrhotite is attacked at the earlier stage of the bioleaching but pyrite remains unaffected. The weak potential of oxidation associated with the decomposition of arsenopyrite and the abundance of arsenite leads to a high grade of elemental sulfur in the bioresidue which consumes the major part of the cyanide during the gold recovery. Furthermore. the arsenite and ferrous produced in solution have to be oxidized to obtain a stable precipitate in the waste products and as outlined in previous works of other authors on this subject the addition of iron to obtain a Fe/As molar ratio at least above 3 is necessary. The agitation equipment used in the pilot unit allowed an optimization study calculation for scaling-up the design and power requirements of an industrial installation. For the pretreatment of a gold refractory As high-graded concentrate the reagent cost has the greatest part in the operating cost of the biooxidation step due to the necessary retreatment of the biosolution. In that conditions the power cost is quite marginal. actually it is significant for the treatment of pure pyrite.

Jan 1, 1991

By A. F. Neil, A. S. Bahaj, P. Watkins, P. M. Hyslop, C. E. Kirby, P. A. B. James

The results of initial work indicate that for 3 of the 5 quarry sands bioleaching reduces the iron oxide content to a commercially acceptable 0.035 wt% Fe20 3, although the time taken to achieve this is 14-22 days against a target leach time of 7 day. Subsequent work has identified strains of Aspergillus niger that are more efficient at producing the array of complexing agents for iron and is yielding excellent and rapid reductions in Fe levels. Potentially, bioleaching offers a more environmentally friendly and energy-efficient process for the removal of Fe from glass-making sands than conventional routes but the production of organic acids by fermentation must be achievable with low-cost substrates such as sugar-beet molasses and lactose permeates for bioleaching to offer a commercially viable alternative to treatment with hot mineral acids. Silica sands usually contain three groups of Fe minerals: iron oxyhydroxides, which comprise crystalline types such as goethite and limonite tending to occur as thin, discontinuous coatings and in cracks and crevices on the surface of the quartz; the assemblage of heavy trace minerals, which include ilmenite, rutile, leucoxene, chromite and haematite, commonly present at levels of 0.1 wt% or less as discrete grains or sometimes cemented to the quartz; and clay minerals cemented in pits on the surface of the quartz and in association with weathered, discrete feldspars. The response of the assemblage of iron minerals to short periods of exposure to hot inorganic acids is broadly related to which group they belong to, those that form coatings being accessible to acid and tending to react rapidly whereas the trace minerals are usually resistant and react very slowly, if at all, and the clays are probably digested. The note reports on a preliminary study of the use of microorganisms to develop an alternative clean technology that will give results comparable to those achievable with hot inorganic mineral acids but with more environmentally acceptable procedures. Various fungi and bacteria produce organic acids when grown in certain media. When these acids come into contact with Fe-stained sand grains the iron is removed as soluble complexes. Several strains of Aspergillus niger have been found to produce organic acids from sugar, predominantly citric, gluconic and oxalic acids, their relative yields depending on cultivation medium and conditions. Sand samples were prepared from five quarries in the UK: Levenseat, Lothian; Redhill, Surrey; King's Lynn, Norfolk; Chelford, Cheshire; and Oakamoor, Staffordshire; ranging in iron oxide content from 0.035 to 0.153 wt% Fe203

Jun 18, 1905

By Jae-Chun Lee, Sadia Ilyas

We investigate the technical feasibility of bioleaching of metals from low grade sulphidic ore. Experiments were conducted with an adapted consortium of Sulfobacillus thermosulfidooxidans and Thermoplasm acidophilum in batch bioreactors (1.5 L), small columns (10 kg ore load, 8 % S0 sludge) and large columns (80 kg ore load, 12 % S0 sludge). Approximately 83 % Ni, 85 % Cu, 55 % Fe and 81 % Zn was leached out in batch mode while 93 % Ni, 89 % Zn, 81 % Cu and 65 % Fe was leached out in column reactors. Bioleaching potential improved by acid pre-leaching, adaptation of microbial consortium and periodic bleeding of effluents.

Jan 1, 2014

By E. G. Noble

The potential for using heterotrophic microorganisms to solubilize manganese from low-grade domestic ores is being assessed at the Reno Research. center, U.S. Bureau of Mines. More than 90 pct of the manganese has been extracted from a variety of oxide ores in shake-flask tests using molasses as a source of .. nutrients for the microorganisms. It has also been demonstrated that high recoveries of manganese can be obtained from a domestic wad ore using column bioleaching. 'l'he isolation and identification of organisms responsible for manganese extraction is discussed, as are the mechanisms responsible for manganese solubilization.

Jan 1, 1991

By H. B. Zhou

The process of bioleaching marmatite using moderately thermophilic bacteria has been studied in this research by comparing marmatite leaching performance of mesophilic bacteria and moderately thermophilic bacteria and valuating the effect of venting capacity on marmatite leaching performance of moderately thermophilic bacteria and the effect of pulp densities on marmatite leaching performance of moderately thermophilic bacteria. The results show that moderately thermophilic bacteria have more advantages over mesophilic bacteria in bioleaching marmatite. Aeration agitation can improve the transfer of O2 and CO2 in solution and promote the growth of bacteria and therefore, enhance the leaching efficiency. With the increase of pulp density, the total leaching amount of valuable metals increased, however, the extraction efficiency decreased due to many rea-sons, like increasing shear force that results in poorly growth condition for bacteria, etc.

Jan 1, 2014

By Z. Y. Lan

Bioleaching of marmatite with various mixed cultures of microorganisms was investigated. The experiments were carried out in shaking flasks with iron-free nutrient and the pulp density of 10% wt/vol at the temperature of 30?. The results show that zinc is preferentially leached before iron. The leaching rate of zinc with the mixed culture of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Leptospirillum ferrooxidans is 62% in 18 days. The leaching rate of zinc with the mixed culture of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans is nearly the same as that with the mixed culture of Acidithiobacillus ferrooxidans and Leptospirillum ferrooxidans (45% versus 50%). SEM and EDX analysis of the leaching residues indicate that a solid product layer composed of iron sulfide, elemental sulfur and jarosite accumulates in the mineral surface and becomes a barrier to the dissolution of marmatite. The bioleaching of marmatite follows the shrinking core product layer model. When the product layer increases and becomes insoluble, the leaching rate is controlled by the diffusion through the product layer and slows down. When the product layer decreases or becomes a porous layer, the leaching rate is controlled by chemical reaction and enhanced dramatically.

Jan 1, 2014

By A. Monballiu, J. A. Martens, Y. W. Chiang, A. Van Audenaerde, R. M. Santos, T. Van Gerven, B. Meesschaert

In this work, bioleaching of non-sulphidic materials by applying chemoheterotrophic bacteria and fungi is studied. It was found that the tested fungus, Aspergillus niger, leached substantially more nickel from olivine than the tested bacterium, Bacillus mucilaginosus. A. niger also outperformed fungal species Humicola grisae and Penicillium chrysogenum in the leaching of olivine. Contrary to traditional acid leaching, the microorganisms leached nickel preferentially over magnesium and iron. On average, a selectivity factor of 2.2 was achieved for nickel compared to iron. This can potentially facilitate postprocessing of the leach liquor and reduce the cost. The impact of ultrasonic conditioning on bioleaching was also tested, and substantial increase in nickel extraction with A. niger was observed. This was credited to an increase in the fungal growth rate, the promotion of particle degradation, and the detachment of the stagnant biofilm around the particles. Furthermore, ultrasonic conditioning enhanced the selectivity of A. niger for nickel, resulting in a selectivity ratio for Ni/Fe equal to 3.5. The chemical resistance of the selected microorganisms to several heavy metal and metalloid (As(III,V), Cd, Cr(III,V), Ni, Pb, Zn) was evaluated. It was found that, with the exception of As(v) and Cd, A. niger has high tolerance to these components. Therefore, this biohydrometallurgical route can potentially be applied to non-sulphidic wastederived materials, such as industrial slags, ashes and sludges.

Jan 1, 2014

By Cheryl Devine, Radhakrishnan Mahadevan, Vladimiros G. Papangelakis, Srinath Garg

The microbial-mediated recovery of valuable metals locked in mining wastes presents an economical alternative to conventional hydrometallurgical processes. The present study investigated the bioleaching response of a low-grade nickeliferous pyrrhotite tailings (0.6 wt% Ni) and an upgraded pyrrhotite tailings (1 wt% Ni) produced by Vale Base Metals in the Sudbury Basin of Ontario, with the ultimate aim of maximizing Ni recovery. The initial part of the study focused on the bacterial adaptive evolution protocol, by repeatedly sub-culturing an indigenous culture from an acid mine drainage site on 2% (w/v) pyrrhotite tailings. The results showed that the extent of Ni dissolution is positively correlated with microbial activity. Microbial profiling revealed A. ferroxidans to be the dominant member of the microbial consortium. The enriched culture was used for optimizing the growth medium by varying the concentration of essential growth nutrients. Finally, an aerated biotic and abiotic leach of a 10% (w/v) upgraded pyrrhotite tailings with and without pH control, showed a higher recovery of Ni in the pH controlled experiments. These results demonstrate that a high concentration of the oxidant Fe3+ in solution promotes the dissolution of Ni. Furthermore, the sulphide conversion into So was found to be higher in the pH controlled experiments (32%), relative to the experiment without pH control (10%).

Jan 1, 2015

By E. Krause, V. G. Papangelakis, S. Garg, R. Mahadevan, E. Edwards

"Bioleaching uses iron and sulfur-oxidizing bacteria to extract base metals from sulfide minerals. As bioleaching involves breaking the iron-sulfur bonds by ferric attack, the biologically mediated rate of iron oxidation is recognized to play a key role in influencing the overall kinetics. In this study, we investigate the catalytic ability of an indigenous AMD culture for oxidizing Fe(II) in solution. This culture was subsequently adapted on pyrrhotite tailings (0.6% Ni) by gradually increasing the solids loading. Results from this study show that such an adaptation reduces the initial lag phase in the biotic oxidation of Fe(II), thereby allowing for a higher Ni extraction when compared to experiments with an un-adapted culture at a similar solids loading. The use of adapted cultures also helps to reduce the residence time for bioleaching of Ni from pyrrhotite tailings. INTRODUCTIONAs the world demand for pure base metals grows; the mining industry is increasingly faced with the burden of processing lower grade ores and mining waste streams in order to recover the occluded metal VALUES ((Watling, 2006). The extractive metallurgy of nickel from sulfide minerals focuses on using a combination of mineral processing, pyrometallurgical (smelting) and hydrometallurgical techniques to extract Ni from its ore bodies.Pyrrhotite (Fe1-xS) is commonly found in association with pentlandite and chalcopyrite in sulfidic Ni ores, such as those found in Sudbury, Ontario, and in Russia, e.g. Norilsk. The vast majority of this pyrrhotite needs to be removed using mineral processing techniques, in order to minimize the Fe and S contents of the concentrates that proceed on to smelting. If all pyrrhotite were left in the concentrate, much more slag and SO2 would be produced, and the smelter throughput would be limited. Beneficiation of Ni sulfide ores leads to the rejection of unwanted constituents as mining wastes, which may still contain extractable amounts of Ni (Edgar Peek et al., 2010)."

Jan 1, 2012

By M. N. Herrera

The bioleaching of mineral sulfide pulps in a bench-scale rotating drum reactor has been studied. The reactor includes horizontal paddles in the inner wall for lifting of the mineral pulp. The performance of the reactor was tested in the bioleaching of a refractory pyritic gold concentrate in the presence of pure Thiobacillus ferrooxidans strain (ATCC 19859). The concentrate was efficiently bioleached at 50% pulp density with the drum operating at 0.5 -1.0 rpm Attempts of bioleaching the concentrate at the same pulp density in conventional stirred reactor showed severe inhibition of bacterial activity. The results demonstrated that the drum reactor allows to operate at much higher pulp densities than the conventional stirred tank reactor. This behavior is explained in terms of an improvement of the bacterial activity due to a better control of the attrition of the mineral particles.

Jan 1, 1997

By Michael L. Free

Bacterial oxidation of an arsenopyritic gold-ore .concentrate was carried out with continuous-flow .operation in 14-liter, mechanically agitated reactors. Samples from the slurry reactors were separated into solid and liquid fractions and analyzed separately to determine the electrochemical rates of sulfide conversion by the bacteria and the rates of direct bacterial oxidation of sulfide. The rates of the individual mechanisms are compared to the overall rates of biooxidation in the continuous-flow slurry reactors. The results show that the commonly hypothesize4 direct and indirect mechanisms of biooxidation both play a significant role in the bacterial oxidation of sulfide ores.

Jan 1, 1991

By Morin D. H., Battaglia-Brunet F., D'Hugues P.

"In the mineral-processing field, bioleaching of metal sulphide concentrates is currently operated in very large mechanically agitated tanks. Such bioreactors have considerable requirements in terms of power consumption for mixing, aeration and heat transfer. The use of slurry bubble column, where the particles are suspended by gas motion as well as by liquid upward flow, could be an alternative to achieve bioleaching in more economical conditions. In order to compare these two techniques, a unit of stirred reactors in cascade and a bubble column were comparatively operated at laboratory scale in the bioleaching of a pyrite concentrate. For both pieces of equipment, experiments were performed not only in batch mode but also in continuous conditions.Bubble column performances were significantly affected by unpredictable technical difficulties met to maintain continuous feeding in the bubble column and by a significant settling of the heavy solid particles. Nonetheless, theoretical kinetic data, extrapolated from results in batch conditions, could be compared to those obtained in continuous tests, in bubble column and in mechanically agitated reactors. These kinetic data were used to evaluate the influence of reactor design and configuration choice on bioleaching performances."

Jan 1, 2003

By Jorge Alberto Soares Tenório, Denise Crocce Romano Espinosa, Solange Kazue Utimura, Amilton Barbosa Botelho, Carlos Gonzalo Alvarez Rosario

In this study, the recycling of waste materials is interesting in the environmental aspect to reduce the amount of waste in land fill and attenuate the pollution of groundwater, air and soil due to the liberation of dangerous elements. Recycling materials is an important issue once it represents a potential source of valuable metals. Waste printed wired boards (PWBs) as a principal part of Waste Electrical and Electronic Equipment (WEEE) and it is a fast growing stream with complex composition. The composite materials from PWBs are ceramic, polymers and metals. Biohydrometallurgical route may offer possibilities to recycle PWBs from discarded computers with the order to recover copper. The extraction of copper from PWBs by bioleaching processeswas studied using a mixed culture of acidophilic bacteria fromacid mine drainage (AMD) and the bacteria Acidithiobacillus ferrooxidans (A. ferrooxidans). The bioleaching experiments were performed in shaker flasks at temperature 30 °C and 170 rpmwith 10% (v/v) inoculum and pulp density of 35 g/L. Themetals concentration was determined by energy dispersiveX-ray Fluorescence (XRF). The results showed that the maximum copper recovery from PWBs using A. ferrooxidans was approximately 89% and with a mixed culture of bacteria from AMD the copper recovery was 92%.

Mar 1, 2017