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By V. Castranova, K. VanDyke

"INTRODUCTION A soluble mediator has been described which is released from activated rabbit leukocytes and induces histamine secretion from rabbit platelets (Barbaro and Zvaifler, 1966; Siraganian and Osler, 1971). This factor has been isolated, characterized and named platelet activating factor, PAF (Bcnveniste et. al., 1972). PAF has since been chemically identified as l-0-alkyl-2-acetyl-sn-glyceryl-3-phosphocholine (Benveniste et. al., 1979; Demopoulous et. al., 1979).PAF is a lipid mediator of broad biological activity. It causes smooth muscle contraction, contraction of lung tissue and decreased FEV 1; while enhancing capillary permeability thus causing edema (Pinkard, 1983). In addition, PAF is a chemoattractant and can lead to inflammation.PAF has been reported to stimulate the release of lysosomal enzymes, leukotriene B,, and lipid metabolites from neutrophils (Smith et. al., 1983; Shaw et. al., 1981; Lin et. al., 1982; O'Flaherty et. al., 1984). The objective of the present investigation was to further characterize PAP-induced stimulation of granulocytes and to develop a bioassay, i.e. chemiluminescence, for screening PAF receptor antagonists and inhibitors of PAP-induced activation of granulocytes. The possible use of the chemiluminescence assay to monitor PAF synthesis by human granulocytes is also described.METHODSBlood was drawn from healthy volunteers into a blood storage bag containing CPD anticoagulant. Granulocytes were partially purified by dextran sedimentation and further purified by centrifugal elutriation as described previously (Jones et. al., 1980). Granulocytes obtained by this procedure were approximately 95 percent pure. The number of granulocytes in this preparation was determined using an electronic cell counter (Coulter Model Z1, Coulter Instrument Co., Hialeah, FL). Isolated granulocytes were used to monitor membrane potential, hydrogen peroxide and superoxide release, oxygen consumption, calcium uptake and, in some cases, chemiluminescence. Studies of PAF receptor antagonists were conducted using neutrophils isolated by density centrifugation. Briefly, blood was layered onto Mono-Poly-Resolving Medium (Flow Laboratories, McLean, VA) and centrifuged at 22 °C and 300 g for 30 min. Neutrophils collected from the second band of this gradient were at least 90 percent pure."

Jan 1, 1989

By Harrison R. Cooper

Composition data is needed as input for executing process control and management of particulate separation for flotation control. While requirements for composition data can be satisfied in many cases by manual sampling and analysis, automatic sampling and assay systems are now viewed as justified for all but the simplest or smallest processes. On-stream X-ray fluorescence analysis (XRFA) has satisfied requirements for the main portion of metalliferous sulfide ore flotation processes as XRFA technology evolved in reliability and sophistication from its beginnings in the late 1950's. In recent years, advanced on-stream analysis technologies have reached levels of development suitable for use in process control to increase efficiencies of important processes in mining and minerals industries such as coal fines flotation and industrial minerals beneficiation (particularly phosphate beneficiation) where XRFA methods have been inapplicable. Recent technical developments pertinent to use of advanced technologies as well as improvements to established on-stream composition analysis methods are discussed.

Jan 1, 1989

By S. S. Gajin

The paper deals with parametric vibrations of an axially loaded pile. The dynamic model is derived by means of Hamilton's principle and reduced to a partial differen­tial equation with variable coefficients and corresponding initial boundary conditions, taking into account the influence of the axial force. By means of Galerkin's method, the partial equation is further reduced to a Matheu-Hill's matrix partial differential equation of the second order. Since the problem of the dynamic stabili­ty has been expressed only for standing piles, the paper analyses in detail two stan­ding piles, one which is restreined, whereas the other is hinged at the toe. In both cases it is assumed that head horisontal displacement is prevented. Using Ruunge-Kutt's method, a computer analysis in the time domain is given for the characteristic parameters and possible boundary conditions. The paper analyses the stationariness of the process, as well as the dependance of the critical excitation frequency upon the pile parameters. The results have been illustrated by respective diagrams.

Jan 1, 1989

By D. Mohan

Soft to medium dense silty and clayey soils are found in many parts of India and conventional friction piles have normally been used for foundations of high rise buildings and other structures on such soils. The author investigated the use of Bored Compaction Piles and these have been used on these soils achieving substantial economies in cost over conventional friction piles. Similarly highly expansive clays, commonly known as Indian Black Cotton Soils occupy about 20 percent of the Indian land area. Short bored underreamed piles have been developed for such soils and a very large construction of various types of buildings and other structures have come up on such piles over the last thirty years in various parts of the country. The paper describes investigations leading to the design and development of both these type of foundations.

Jan 1, 1989

By W. L. Cornell

The Missouri Pb ores are the only domestic Co resource being mined and processed for other metals; therefore, they present a viable short-term opportunity for Co production. Lead, zinc, and copper concentrates are produced from the ore. The Cu concentrate can contain up to 30 pct Co and the Pb concentrate up to 15 pct Co. Since Co is detrimental to the processing of the Zn concentrate; the remainder of the Co is rejected to the tailings. The tailings can contain as much as 50 pet Co originally present in the mined ore. Researchers at the U.S. Bureau of Mines have successfully tested on a continuous basis a process that recovers a bulk sulfide concentrate from mill tailings. The concentrate contains up to one-half of the Co and from 50 to 90 pct of the Pb, Zn, and Cu. Concentrate weight represents 10 pet or less of the total tailings.

Jan 1, 1989

By Leszek Hoser

Polycrystalline oxide superconductors that have the perovskite structure, that are oxygen deficient, and that have compositions based. on the so-called. 1-2-3 (YBa2Cu3O7) compound, were investigated.. The materials exhibit a high critical transition to superconductivity at temperatures near 90oK. It was determined that maintaining the oxygen content within the correct range in connection with an appropriate amount of CU ions in the crystal lattice leads to high-critical temperature (Tc:) superconducting properties. High-Tc: superconductors with a critical temperature in the range of 100°K were developed. Further investigations, based. on the theoretical examinations of the origin of superconductivity, have been performed and are discussed..

Jan 1, 1989

By B. R. Mohal, S. Chander

"The adsorption and wetting behavior of freshly ground and aged coals has been determined in the presence of a nonionic surfactant. The amount of surfactant adsorption changes with the concentration and ""age"" of the coal. The freshly ground coal has smaller amounts of adsorption at lower surfactant concentrations than the ""aged"" coal whereas at large surfactant concentrations, the order is reversed . In the latter case, more surfactant is adsorbed on fresh coal than on the ""aged"" coal . . It is proposed that at low concentrations, the surfactant adsorbs through both hydrophobic and hydrophilic interactions with the molecule lying parallel to the· surface, whereas at higher concentrations surface micelles form at the hydrophobic sites. The wetting behavior correlates well with the adsorption behavior.INTRODUCTIONNonionic surfactants have many applications in the coal and mineral processing industry. They can be used as dispersing agents to modulate flotation, flocculation and filtration of particulates (1). They can also be used to increase the wettability of hydrophobic solids such as coal and can hence be used as dust suppressants (2,3). Although the adsorption of representative nonionic surfactants such as Triton N-101 (an ethoxylated nonylphenol with 9.5 moles of ~thylene oxide and HLB equal to 13.4) have been studied extensively on substrates such as calcium carbonate (4), silica (5) carbon black (6) and graphite (7), very few studies are available for coals. Coals differ from other substrates with respect to two important characteristics: 1) coal are porous and 2) coals consist of a patchwork of hydrophobic and hydrophilic sites (8). Since the adsorption characteristics of a surfactant are influenced significantly by the nature of the substrate the characteristics of the coals must be taken into consideration."

Jan 1, 1989

By N. S. Dalal, X. Shi

"It has been recently suggested that the exceptionally high antitumor and antibacterial activity of natural fredericamycin A (FMA) is related to its ability to spontaneously generate the superoxide anion (02-) and hydroxyl (""OH) radicals in aerobic solutions [Hilton, B. D., Misra, R., & Zweier, J. L. (1986) Biochemistry 25, 5533]. With a view to understand the mechanistic details, attempts were made to reproduce earlier electron spin resonance (ESR) evidence for the oxygenated free radical formation in well-aerated solutions of natural FMA in dimethyl sulfoxide and dilute H 20 2• Little or no evidence was obtained for the formation of the 0 2- and methoxy (""OCH3) radicals, while the detected formation of the 'OH and methyl ('CH3) radicals was attributable largely to mechanisms not involving FMA. These results thus reopen the T question regarding the mechanism of its exceptionally high tumoricidal-bacteriocidal activity. This paper reports on an electron spin resonance (ESR) investigation of the formation of oxygenated free radicals in solutions of natural fredericamycin A (abbreviated as FMA) which has recently been reported to be a highly potent anti¬tumor antibiotic (Pandey et al., 1981; Warnick-Pickle et al., 1981). The impetus for this undertaking was provided by recent reports (Hilton et al., 1986; Stinson, 1986) suggesting that the strong tumoricidal activity with high therapeutic—toxic index of FMA was related to its ability for the spontaneous generation of superoxide anion (02-) and hydroxyl (OH) radicals. For example, the suggested mechanism for the formation of 02- by FMA was (Hilton et al., 1986)FMA-OH + O2 FMA-O + H+ + 02-Here FMA—OH is simply the FMA molecule emphasizing the —OH moiety which becomes FMA-O, the free radical rep¬resenting the oxidized form of the drug. While some other highly effective anticancer drugs in current clinical application, such as bleomycin, are also thought to exert their antitumor effects via the formation of reactive drug-based or oxygenated free radicals (Carmichael et al., 1985; Gianni et al., 1983), FMA was considered unique in that only in this case the free radical formation was thought to be spontaneous, rather than through a metal- or an enzyme-activated process (Hilton et al., 1986; Stinson, 1986). Thus it was suggested (Hilton et al., 1986; Stinson, 1986) that FMA could be the forerunner of a new class of anticancer drugs. However, we noted that while the hypothesis seemed plausible, the reported ESR spectral evidence appeared to be ambiguous. Because of the high significance of the earlier conclusions (Stinson, 1986), we undertook a systematic ESR study of the radical formation in FMA solutions, and as discussed below, our conclusions disagree with those reported earlier."

Jan 1, 1989

By Cornwell P. J

The Pajingo ore bodies consist mainly of auriferous quartz veins hosted by a suite of hydrothermally altered andesitic pyroclastics, volcanics and epiclastic sediments. Scott lode, the main economic deposit in the area, lies in a zone of major structural disruption and contortion. High grade gold and silver mineralisation is selectively mined by open pit methods. Standard grade control techniques and extraction methods were adapted to minimise mining dilution and to maximise metal recovery. Basic blending of grade ranges at the crusher has produced an average 10 gram/tonne gold head grade. The estimation of ore reserves calculated by both standard polygonal and geostatistical methods proved acceptable. Mineable reserves when compared to in pit reserves defined by blast hole drilling, indicate a decrease in tonnes and an increase in the grade resulting in an acceptable comparison of the total ounces of contained gold. The ore reconciliation of mined against milled ounces indicates that both ore reserve and grade control models underestimate the metal content in the orebody. Sampling techniques are being examined in an attempt to reduce this discrepancy.

Jan 1, 1989

By N. S. Dalal, B. Jafarl, M. M. Suryan, V. Vallyathan

"The biologic events leading to coal workers' pneumoconiosis (C'WP) are not yet fully understood. There is no good correlation between toxicity studies or animal models with human epidemiologic and pathologic data. Several studies have shown that the severity and prevalence of the CWP in miners correlate strongly with the rank and type of the coal mined. However this has not been found to be the case with laboratory animal models or in vitro studies. In 1980 Russian scientists Artemov and Resnik suggested that the discrepancy may be related to the excessive amount of short-lived free radicals present in freshly generated coal mine dust. Because of their short life, such free radicals would be absent in the ""stale"" coal dust used in most animal experiments. To test this hypothesis we have undertaken a systematic study of the generation, reactivity with oxygen and biological significance of free radicals in coal dust.Electron spin resonance (ESR) was used as a technique for the direct detection of radical production and kinetics of decay. ESR signals from the newly generated radicals decreased with time on exposure to oxygen or when stored in biological buffers at a rapid rate. In vitro biological tests were carried out which suggest that the reaction between the newly generated radicals and oxygen plays a significant role in the sample's cytotoxicity.Materials and MethodsESR measurements were made at X-band (~9.7 GHz) using a computer-controlled Bruker ER200D ESR spectrometer. 11te two coals studied in detail were obtained from the Pennsylvania State University Coal Depository. These were, a bituminous sample, PSOC-1172 (72% carbon) and an anthracite sample, PSOC 867 (95% carbon). Coal particles of several millimeter (mm) dimensions were evacuated to a pressure of ~10-3 mm Hg typically overnight. Without exposure to air, these particles were ground in an agate mortar with an agate pestle for about 30 minutes and then sieved through a 20 \1111 mesh filter before ESR measurements or cytotoxicity studies. As an index of cytotoxicity, hemolysis was measured according to the method of Harrington, with minor modifications. Sheep erythrocytes (2%) were incubated with test samples at 37°C for one hour, and the hemoglobin released was quantitatively estimated photometrically. Additionally, release of alveolar macrophage enzyme lactate dehydrogenase (LOH) was estimated after incubation of the macrophages with the coal particles at different time intervals after the grinding. Enzyme activity was assayed according to the method of Wrobleski and LaDue and expressed as percent of enzyme released in 2 hours per million cells with 1 mg of coal."

Jan 1, 1989

By Randal J. Barnes

The nonlinear network optimization algorithm developed in this paper solves the following mine ventilation design problem: find the location and size of all fans and regulators which minimize the overall system power consumption while satisfying all physical, legal, and operational constraints. This work includes the formulation of the problem within the framework of mathematical optimization, the development of the computational theory, and the creation of an efficient solution methodology. The algorithm has been implemented on a microcomputer, and a small but representative example is included. The algorithm presented in this paper has four limitations. First, the optimization process is for a single point in time: the dynamic nature of a mine ventilation system in an evolving mine is not directly addressed. Second, the topology of the ventilation network is required input information. The mathematical algorithm does not optimize the size or location of the mine ventilation airways; however, the post optimality analysis techniques can be used to aid in topological decisions. Third, the problem is formulated using volumetric flow; thus, heat and moisture transfer are ignored. Fourth, the capital cost of ventilation equipment is not included in the formulation.

Jan 1, 1989

By V. Srajer

The loading and hauling equipment simulation model was developed to satisfy, the requirements and need of the mining industry in western Canada. It is expected the model will be used to determine the' impact of various combina¬tion of loading and hauling equipment, company policies and maintenance procedures, haulage requirement, to evaluate possibilities, of the use of truck monitoring and dispatch systems, and to access loading and hauling equipment productivities. To evaluate the model, and to access its capabilities in addressing the above requirements, the model was validated. The validation was based on the following: 1. Running the model in-house to evaluate its structure, 2. Running the "test case" by timing models developed elsewhere to compare the results of simulation, 3. Soliciting of model critique from outside agencies, and 4. Comparison of calculated data with real time study data. The presented paper provide overview of the validation processes and summarize" results obtained during comparison and evaluation studies.

Jan 1, 1989

By Ari JokiIaakso

Three different sulphidic raw materials were treated in a laboratory scale laminar flow fur-nace simulating suspension smelting conditions. The materials were a pure chalcopyrite concentrate, a high-antimony concentrate (tetrahedritic), and crushed copper mattes. The gas preheating temperatures were 500 -1 100°C ana the atmospheres ranged from N2 to N2 -75 % O2, Two particle size fractions were used for the concentrates with x = 20 -38 um and x = 62 -74 um, and three for the granulated copper mattes with x < 37 um, x = 62 -88 um, and x = 125 -177 um. Scanning electron microscopy combined with energy dispersive analysis and backscattered electron analysis was used for determining the morphology and the mineralogy of the reacted particles. Fragmentation of the particles were observed in all materials during the flash oxidation, the conditions depending on the iron and sulphur content and the original particle size fraction of the material. The melting and/or reaction stages of the particles in different conditions were studied from powder samples. Polished sections were examined in order to follow the development of porosity and the mineralogy of the particles.

Jan 1, 1989

By Robert C. White, Kurt W. Keskimaki

The Henderson Mine is a large underground panel cave molybdenum mine located near Empire, Colorado. Diesel equipment is employed for all development work, as well as production ore handling. Due to fluctuating demand for molybdenum, production rates have varied from 16,000 to 32,000 metric tons per day (18,000 to 36,000 short tons per day) over the last four years. In order to obtain the maximum air flow for minimum power cost the Henderson mine has completed the following modifications to the ventilation system: removal of large intake fans, elimination of underground booster fans, shutdown of tunnel exhaust fans, completion of the number 5 exhaust ventilation shaft, and use of partially debladed main exhaust fans. These changes have reduced the annual cost of mine ventilation at the Henderson Mine by over 80 per cent, a very critical cost savings in today&apos;s competitive molybdenum marketplace.

Jan 1, 1989

By Robert S. Hedin, David M. Hyman

Acid mine drainage (AMD) is a common water pollution problem on active and abandoned coal mine sites in the eastern United States. AMD forms when surface mining brings unweathered pyrite-containing rocks to the surface or when deep mining allows oxygen to contact buried pyritic strata. In the absence of neutralizing compounds, the drainage that results can be extremely acidic and contaminated with dissolved iron, manganese, and sulfate. Drainages with pH < 3.0 and concentrations of sulfate greater than 1,000 mg/L, iron greater than 50 mg/L, and manganese greater than 10 mg/L are common. Where water flows through alkaline materials (such as limestone) before surfacing, the drainage is less acidic and occasionally circumneutral, but it can still contain high concentrations of sulfate and metals. Current water quality standards in the United States require that mine discharges have a pH between 6 and 9, total iron concentration less than 3.0 mg/L, and manganese less than 2.0 mg/L. At thousands of active and inactive mine sites, drainage does not meet these standards and is being treated before discharge by the mining company. At thousands of other sites, which were abandoned prior to the enactment of water pollution laws or were operated by companies that have gone bankrupt, untreated AMD is polluting receiving water systems. The standard mine drainage treatment system involves the addition of a1 kal ine chemicals to the water, which raises the pH and cause metals to precipitate in a settling pond. These systems are expensive, often costing tens or hundreds of thousands of dollars per year for chemicals, operation, maintenance, and disposal of the metal- laden sludge. Because the drainage on many sites will likely be contaminated for decades, there is financial incentive to find alternative water treatment systems. In the last five years, many mining companies and engineering firms have experimented with the construction of wetland treatment systems. Very few of the several hundred wetland systems that have been built are performing well enough to justify total abandonment of chemical treatment. Nonetheless, systems continue to be built because the mining companies have found that wetlands can reduce their AMD treatment costs. It is also their hope that further research and development will eventually result in systems that will completely rep1 ace chemical treatment and offer long term performance at minimal cost. The Bureau of Mines has been actively involved, from a research perspective, with this approach to AMD treatment since its inception. In this paper, the status of constructed wetland technology is discussed with respect to the construction and performance of systems, chemical and biological processes that affect AMD chemistry within constructed wetlands, and the future of this technology as perceived by the Bureau of Mines. ORIGINAL OBSERVATIONS The constructed wetland concept has its roots in observations of natural Sphaqnum peat wetlands that received acid mine drainage and, instead of being adversely affected, appeared to cause an amelioration of the polluted water (Huntsman et al. 1978; Wieder and Lang 1982). These observations instigated the idea that wet1 and systems might be used for the intentional treatment of mine drainage. Because the discharge of AMD into a natural wetland is prohibited in the U.S. by several laws, it has been necessary to construct wetlands which act solely as water treatment systems. Initially, most wetland research and construction efforts mimicked the original observations by utilizing Sphaqnum moss and peat. Despite promising laboratory results (Kleinmann et al. 1983; Tarleton et al. 1984; Burris et al. 1984; Gerber et al. 1985), virtually all field tests of Sphasnum-dominated constructed wetlands failed to provide sufficient water treatment for more than several months. Sphasnum proved quite sensitive to the stresses associated with transplanting, abrupt changes in water chemistry, excessive or insufficient water depth, and

Jan 1, 1989

By Donna L. Anderson

This U.S. Bureau of Mines report describes a system to determine the position and heading of a continuous mining machine during the maneuvers required at the face area of a mine. The system consists of commercially available angular position sensors and a computer interface developed and tested at the Bureau. The sensors employed are laser-based scanning devices that report the angular location of retroreflective targets within their 90° field-of-view. The targets are mounted on the continuous mining machine; the laser scanners are mounted on an external reference structure. The computer interface consists of a single-board computer-controller programmed to gather and process the angular position information, and to calculate the machine&apos;s position and heading relative to the reference structure. A sensor fusion algorithm will be incorporated into the computer interface programming to increase the accuracy and reliability of the system.

Jan 1, 1989

By Fernando N, Guang LX

Brown coal in Victoria in spite of its substantial reserves is not utilized greatly because of its high moisture content(-65% w/w). Transportation and handling costs of raw brown coal, on an available energy basis are so expensive that 93% of production is burnt for electricity generation on or adjacent to the coal deposit in Victoria. With direct firing of moist brown coal in power stations, approximately 20% of the gross calorific value of the fuse is used to evaporate the water contained in the coal. This energy is lost as vapour with the flue gas which is vented to the atmosphere. Also there is a 25% increase in flue gas volume and the size and cost of most of the units through which it passes must be appropriately larger. Since there are large quantities of water present the flame temperature is significantly reduced. For zero excess air and no air pre-heating the theoretical flame temperatures are: 609&apos;o wow moisture 1450¦C 40% ª ª ª 1790¦C ZOO ª ª ª 1990¦C As a result of this there is a reduction in radiant heat transfer leading to a requirement for a two fold increase in radiant tube area. A boiler of much greater size and cost is thus required to house the additional tubes. Figure 1 illustrates the relative sizes of a boiler fired with 60% moisture brown coal with a boiler or similar output fed with a British bituminous coal, 14% moisture.

Jan 1, 1989

By John J. Reed

The 1945 fire at the Braden (El Teniente) Copper Mine in Chile took 354 lives, and ranks as the most lethal metal mine fire in history. The mine was developed in the wall of a canyon over 900 m (3,000 ft) deep. Principal entrance was through a main haulage adit at the canyon bottom, and up through two internal shafts. Mining was by block caving from the top of the orebody with most of about 900 men on shift working in three production areas. Ore was transferred by gravity to the haulage level through a complex system of ore passes with associated ladderways. Normal mine ventilation was incast through numerous level portals and up- cast through the shafts and raises. The fire was confined to a maintenance shop just inside the portal of the lower production level. Smoke, gases and flammable vapors were drawn into the mine, and a subsequent explosion of the gases destroyed an air door and injected carbon monoxide into the workings. Falling water in a drainage raise near the fire made it downcast, which carried gases down to all the lower levels where they were drawn into the levels and then up connecting raises. Miners attempting to escape by climbing down the ladderways from the production levels were met and killed by the rising gases.

Jan 1, 1989

By J. M. Cases

Diffuse - reflectance Fourier - transform infrared spectroscopy, Hallimond tube flotation and microelectrophoresis have been utilized to investigate the reactions involved in the adsorption-abstraction of K-amylxanthate on finely ground galena and pyrite. In order to work under well-controlled conditions both minerals were wet ground in a specially built laboratory stainless steel or iron mill. Four surface products were found to be formed on galena: monocoordinate lead xanthate, non-stoichiometric lead xanthate, dixanthogen, and amyl carbonate disulphide. Only dixanthogen was observed on pyrite after using stainless steel rods, while ferric iron xanthate was found when iron rods were utilized.

Jan 1, 1989

"INTRODUCTIONGold tailings retreatment plants have been operated for many years throughout the world. Several large tailings retreatment facilities have been operated profitably in South Africa since 1976 and there are tailings retreatment operations in Canada currently in production with more in the feasibility stages.The Timmins Gold Tailings Project of ERG Resources Inc. was designed to treat 8,000,000 tonnes per annum of gold tailings for an eight month operating season, and commissioning of the operation began in July 1988. The payback period for this project is about 3 years which corresponds to an IRR of 30%.This report summarizes the metallurgical characteristics of the reclaim tailings, describes the process flowsheet which was developed to enable ERG to economically recover gold from the Timmins area tailings, and reviews commissioning activities."

Jan 1, 1989